Splenic artery ligation improves remnant liver function in partially hepatectomized rats with ischemia/reperfusion injury
Splenic artery ligation improves remnant liver function in partially hepatectomized rats with ischemia/reperfusion injury
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DOI：
10.1111/j.1478-3231.2006.01432.x
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发表时间：
2007-04-01
影响因子：
6.7
通讯作者：
Horikawa, Saburo
中科院分区：
文献类型：
--
作者：
Ito, Koji;Ozasa, Hisashi;Horikawa, Saburo
在肝脏切除术中，经常使用暂时阻断肝十二指肠韧带（Pringle手法）。然而，该操作导致严重的缺血/再灌注损伤的残肝。为探讨脾动脉结扎对Pringle法肝部分切除大鼠肝功能的影响，在2/3肝部分切除前30 min行Pringle法肝部分切除术。脾动脉结扎前进行普林格尔手法。脾动脉结扎的有效性进行了评估的生存，血清丙氨酸氨基转移酶（ALT），天冬氨酸氨基转移酶（AST），残余肝脏重量的恢复，和portalpressure.On第3天，动物存活率为4的12只大鼠部分肝切除大鼠与Pringle手法和10的12只大鼠脾动脉结扎治疗部分肝切除大鼠与Pringle手法。单独的三分之二部分肝切除术或脾动脉结扎本身对生存率没有任何影响。与肝部分切除大鼠相比，脾动脉结扎动物血清AST和ALT水平较低，残肝重量恢复较高。脾动脉结扎可明显降低过度肝切除大鼠的门静脉压力，降低病死率;脾动脉结扎可改善Pringle法部分肝切除大鼠和过度肝切除大鼠的残余肝功能。改善可以至少通过降低门静脉压力来介导。
In liver resection, the temporary occlusion of the hepatoduodenal ligament (Pringle maneuver) is often used. However, the maneuver causes severe ischemia/reperfusion injury in the remnant liver. Our aim was to investigate the effects of splenic artery ligation on the liver function in partially hepatectomized rat with the Pringle maneuver.The Pringle maneuver was conducted for 30 min just before a two-thirds partial hepatectomy. Splenic artery ligation was performed before the Pringle maneuver. The efficacy of splenic artery ligation was assessed by survival, serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), recovery of remnant liver weight, and portal pressure.On day 3, animal survival was four rats of 12 in partially hepatectomized rats with the Pringle maneuver and 10 rats of 12 in the splenic artery ligation-treated partially hepatectomized rats with the Pringle maneuver. A two-thirds partial hepatectomy alone or splenic artery ligation itself did not show any effects on the survival. Compared with partially hepatectomized rats with the Pringle maneuver, splenic artery-ligated animals had lower serum AST and ALT levels, and higher recovery of remnant liver weight. Splenic artery ligation significantly reduced the portal pressure and also decreased the fatality in excessively hepatectomized rats.Splenic artery ligation ameliorated the remnant liver function in partially hepatectomized rats with the Pringle maneuver and excessively hepatectomized rats. The amelioration may be mediated at least by decreasing portal pressure.