Identification, chromosomal mapping and conserved synteny of porcine Argonaute family of genes
Identification, chromosomal mapping and conserved synteny of porcine Argonaute family of genes
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DOI：
10.1007/s10709-010-9462-z
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发表时间：
2010-05
期刊：
影响因子：
1.5
通讯作者：
Xiaoping Zhou;Heng Guo;Ke Chen;Hanhua Cheng;R. Zhou
中科院分区：
文献类型：
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作者：
Xiaoping Zhou;Heng Guo;Ke Chen;Hanhua Cheng;R. Zhou
Argonaute蛋白是新近发现的进化上保守的蛋白家族，有两个亚家族Ago和Piwi，在小RNA通路中起重要作用。大多数物种的基因组中有8个Argonaute成员，从1到27不等。本文报道了猪Argonaute基因的6个序列，其中Ago亚家族的4个成员（Ago 1、Ago 2、Ago 3和Ago 4）和Piwi亚家族的2个成员（Piwil 1和Piwil 2），分别编码857、860、860、861、861和985个氨基酸。系统进化分析表明，猪Ago和Piwi基因与哺乳动物Argonaute基因的相关分支聚在一起。猪Ago 4-Ago 1-Ago 3基因位于6号染色体的p12，而Ago 2位于4号染色体的p15。猪Piwil 1和Piwil 2一起映射到14号染色体上，分别位于q14和q11处。通过对Argonaute成员染色体定位的比较分析，发现Ago 4-Ago 1-Ago 3连锁群及其邻近基因在鸡到哺乳动物的进化过程中是保守的。从鱼类到哺乳动物，Piwil 1和Piwil 2基因被分离到不同的染色体上，除了在猪和棘鱼中的这种趋势之外，表明这两个基因在物种形成过程中重组在一起或不分离的相反趋势。进一步的表达分析显示Ago成员的普遍表达模式，相反，Piwimember的性腺中的限制性表达模式，表明猪Argonaute基因的不同潜在作用。
The Argonaute proteins are recently identified and evolutionarily conserved family with two subfamilies Ago and Piwi, which play important roles in small RNA pathways. Most species have eight Argonaute members in their genomes, ranging from 1 to 27. Here we report identification of six Argonaute genes in pig, four members of the Ago subfamily (Ago1,Ago2,Ago3andAgo4) and two members of the Piwi subfamily (Piwil1andPiwil2), which were predicted to encode proteins of 857, 860, 860, 861, 861 and 985 amino acids, respectively. Phylogenetic analysis showed that the porcine Ago and Piwi genes were clustered into relevant branch of mammalian Argonaute members. The porcineAgo4-Ago1-Ago3genes are linked together at the p12 of the chromosome 6, whileAgo2is located at the p15 of the chromosome 4. The porcinePiwil1andPiwil2are mapped together onto the chromosome 14, at the q14 and q11 respectively. Comparatively mapping of the Argonaute members on chromosomes showed that linkage group of theAgo4-Ago1-Ago3and several neighborhood genes is evolutionarily conserved from chicken to mammals. The genesPiwil1andPiwil2are separated onto different chromosomes from fish to mammals, with exception to this tendency in both pig and stickleback, indicating an opposite tendency of recombination together or non-disjunction of these two genes during speciation. Further expression analysis showed an ubiquitous expression pattern of Ago members, oppositely a restricted expression pattern in gonads of thePiwimembers, suggesting distinct potential roles of the porcine Argonaute genes.