Transcriptome comparison between inactivated and activated ovaries of the honey bee Apis mellifera L.
Transcriptome comparison between inactivated and activated ovaries of the honey bee Apis mellifera L.
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DOI：
10.1111/imb.12114
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发表时间：
2014-10-01
影响因子：
2.6
通讯作者：
Hu, F.
中科院分区：
文献类型：
--
作者：
Niu, D.;Zheng, H.;Hu, F.
卵巢活动不仅影响生育力，而且还参与调节雌性蜜蜂的生殖和行为等级之间的分工。为了筛选与卵巢活动相关的候选基因，我们采用高通量RNA测序技术比较了蜂王和工蜂卵巢失活和激活后的基因表达谱。在未交配蜂王和交配蜂王卵巢间共检测到1615个差异表达基因，在激活和失活的工蜂卵巢间共检测到5300多个差异表达基因。5个文库中DEG的交叉分析表明，一组相似的基因（824）参与了蜂王和工蜂的卵巢激活。这些DEG主要与细胞、细胞和细胞部分、结合、生物调节和代谢过程有关。此外，超过1000个DEG与京都基因和基因组途径百科全书的230多个组成部分相关，包括25个信号传导途径。通过实时定量PCR证实了RNA测序结果的可靠性。我们的研究结果提供了新的见解参与卵巢激活和生殖分工的分子机制。
Ovarian activity not only influences fertility, but is also involved with the regulation of division of labour between reproductive and behavioural castes of female honey bees. In order to identify candidate genes associated with ovarian activity, we compared the gene expression patterns between inactivated and activated ovaries of queens and workers by means of high-throughput RNA-sequencing technology. A total of 1615 differentially expressed genes (DEGs) was detected between ovaries of virgin and mated queens, and more than 5300 DEGs were detected between inactivated and activated worker ovaries. Intersection analysis of DEGs amongst five libraries revealed that a similar set of genes (824) participated in the ovary activation of both queens and workers. A large number of these DEGs were predominantly related to cellular, cell and cell part, binding, biological regulation and metabolic processes. In addition, over 1000 DEGs were linked to more than 230 components of Kyoto Encyclopedia of Genes and Genomes pathways, including 25 signalling pathways. The reliability of the RNA-sequencing results was confirmed by means of quantitative real-time PCR. Our results provide new insights into the molecular mechanisms involved in ovary activation and reproductive division of labour.