SEQUENTIAL-CHANGES IN THE EXPRESSION OF MITOCHONDRIAL PROTEIN MESSENGER-RNA DURING THE DEVELOPMENT OF BROWN ADIPOSE-TISSUE IN BOVINE AND OVINE SPECIES - SUDDEN OCCURRENCE OF UNCOUPLING PROTEIN MESSENGER-RNA DURING EMBRYOGENESIS AND ITS DISAPPEARANCE AFTER BIRTH
SEQUENTIAL-CHANGES IN THE EXPRESSION OF MITOCHONDRIAL PROTEIN MESSENGER-RNA DURING THE DEVELOPMENT OF BROWN ADIPOSE-TISSUE IN BOVINE AND OVINE SPECIES - SUDDEN OCCURRENCE OF UNCOUPLING PROTEIN MESSENGER-RNA DURING EMBRYOGENESIS AND ITS DISAPPEARANCE AFTER BIRTH
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DOI：
10.1042/bj2570665
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发表时间：
1989-02-01
影响因子：
4.1
通讯作者：
RICQUIER, D
中科院分区：
文献类型：
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作者：
CASTEILLA, L;CHAMPIGNY, O;RICQUIER, D
从牛和羊胎儿和新生儿的不同部位获得脂肪组织样本。分别用牛cDNA和羊基因组探针对解偶联蛋白（UCP）、细胞色素c氧化酶III亚基和IV亚基cDNA以及ADP/ATP载体cDNA进行分离和分析。首次在胎牛和绵羊脂肪组织中对UCP mRNA进行了表征。其出现时间晚于细胞色素c氧化酶亚基III mRNA，并在出生时显著增加（10倍）。ADP/ATP载体mRNA的表达水平较低，但在出生时也增加了10倍。结果表明，除皮下组织外，所有牛脂肪组织的UCP mRNA均在出生时达到最高水平。它很快就消失了，在出生两天后就无法检测到。在新生羔羊中也观察到类似的变异。ADP/ATP载体mRNA与UCP mRNA表达模式相同；虽然出生后两天还会有轻微的表情，但很快就消失了。只有细胞色素c氧化酶亚基III和IV的mrna在出生后第一周保持相同水平。根据这些数据和文献报道的观察结果，提出了体内棕色脂肪细胞发育的一系列事件。出生后不久，反刍动物肾周脂肪组织仍含有典型棕色脂肪组织形态的线粒体和高水平的细胞色素c氧化酶mRNA，但缺乏UCP mRNA。它还能被认为是棕色脂肪吗？反刍动物似乎是研究大型动物棕色脂肪组织分化及其可能转化为白色脂肪的有吸引力的模型。
Samples of adipose tissue were obtained from different sites in bovine and ovine foetuses and newborns. RNA was isolated and analysed using bovine cDNA and ovine genomic probe for uncoupling protein (UCP), cDNA for subunits III and IV of cytochrome c oxidase and cDNA for ADP/ATP carrier. UCP mRNA was characterized for the first time in foetal bovine and ovine adipose tissue. It appeared later than mRNA of cytochrome c oxidase subunit III, and increased dramatically at birth (10-fold). ADP/ATP carrier mRNA was expressed at a lower level but also increased 10-fold at birth. It was demonstrated that UCP mRNA reached its highest level at birth in all bovine adipose tissues studied, except subcutaneous tissue. It disappeared quickly afterwards, being no longer detectable two days after birth. Similar variations were observed in newborn lambs. ADP/ATP carrier mRNA showed the same pattern of expression as UCP mRNA; although it was still lightly expressed two days after birth, it disappeared soon afterwards. Only mRNAs for cytochrome c oxidase subunits III and IV remained at the same level during the first postnatal week. On the basis of these data and of observations reported in the literature a sequence of events for the development of brown adipose cells in vivo is proposed. Soon after birth the perirenal adipose tissue of ruminants, which still contains mitochondria of typical brown adipose tissue morphology and high levels of cytochrome c oxidase mRNA, lacks UCP mRNA. Can it still be considered as brown fat? Ruminant species appear to be attractive models to study both the differentiation of brown adipose tissue and its possible conversion to white fat in large animals.