RAPID CONFIRMATION AND REVISION OF THE PRIMARY STRUCTURE OF BOVINE SERUM-ALBUMIN BY ESIMS AND FRIT-FAB LC MS
RAPID CONFIRMATION AND REVISION OF THE PRIMARY STRUCTURE OF BOVINE SERUM-ALBUMIN BY ESIMS AND FRIT-FAB LC MS
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DOI：
10.1016/s0006-291x(05)80083-x
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发表时间：
1990-12-14
影响因子：
3.1
通讯作者：
FUKUHARA, K
中科院分区：
文献类型：
--
作者：
HIRAYAMA, K;AKASHI, S;FUKUHARA, K
根据电喷雾质谱（ESIMS）测定的牛血清白蛋白（BSA）分子量，推测其氨基酸序列不正确。通过快速原子轰击质谱/液相色谱（Frit-FAB LC/MS）、自动Edman降解和串联质谱（MS）的组合，快速发现在第156位的位置缺少酪氨酸残基。结果表明，BSA由583个氨基酸残基组成，其平均分子量不是66267.1，并将其校正为66430.3。此外，通过使用自动Edman降解方法将第94位和第95位的氨基酸序列校正为-QE-。
Incorrectness of the amino acid sequence of bovine serum albumin (BSA) was suggested from the observed molecular weight of BSA obtained by electrospray ionization mass spectrometry (ESIMS). Lack of a tyrosine residue in the position of 156th was found rapidly, by the combination of frit-fast atom bombardment mass spectrometry/liquid chromatography (Frit-FAB LC/MS), automated Edman degradation and tandem mass spectrometry (MS). Then it turned out that BSA is composed of 583 amino acid residues, and that its average molecular weight is not 66267.1, and it is corrected to 66430.3. Moreover the amino acid sequence of the positions of 94th and 95th was corrected to-QE- by using automated Edman degradation method.