Expression or aromatase cytochrome P450 protein and messenger ribonucleic acid in human endometriotic and adenomyotic tissues but not in normal endometrium
Expression or aromatase cytochrome P450 protein and messenger ribonucleic acid in human endometriotic and adenomyotic tissues but not in normal endometrium
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DOI：
10.1095/biolreprod57.3.514
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发表时间：
1997-09-01
影响因子：
3.6
通讯作者：
Honjo, H
中科院分区：
文献类型：
--
作者：
Kitawaki, J;Noguchi, T;Honjo, H
为了确定子宫内膜异位症或腺肌病组织、子宫内膜异位症或子宫腺肌症患者的异位子宫内膜和正常子宫内膜中是否存在局部雌激素产生，我们对组织标本进行了免疫组化、催化活性和芳香化酶细胞色素P450(P450(arom)) mRNA表达的检测。P450（arom）免疫组织化学定位于子宫内膜异位症和腺肌病组织的腺细胞和异位子宫内膜的细胞质中，而雌激素受体和孕激素受体定位于腺细胞和基质的细胞核中。加入达那唑、芳香酶抑制剂和抗人胎盘P450（芳香）单克隆抗体（mAb3-2C2）后，腺肌病组织微粒体部分的芳香酶活性被抑制，其抑制方式与在其他人体组织中的抑制方式相似。逆转录聚合酶链反应和Southern blot分析也显示P450(arom) mRNA在这些组织中表达。然而，在没有其他妇科疾病的宫颈原位癌的正常月经妇女的子宫内膜标本中均未检测到P450（arom）蛋白活性和mRNA。这些结果提示，子宫内膜异位症和腺肌病组织在局部水平上产生雌激素，雌激素可能通过与雌激素受体相互作用参与组织生长。
To determine whether local estrogen production lakes place in endometriotic or adenomyotic tissues, in eutopic endometrium from patients with endometriosis or adenomyosis, and in normal endometrium, tissue specimens were examined try immunohistochemistry, catalytic activity, and mRNA expression for aromatase cytochrome P450 (P450(arom)). P450(arom) was immunohistochemically localized in the cytoplasm of glandular cells of endometriotic and adenomyotic tissues, and of eutopic endometrium from patients with the respective diseases, whereas estrogen receptors and progesterone receptors were localized in the nuclei of the glandular cells and stroma. Aromatase activity in the microsomal fraction of adenomyotic tissues was inhibited by the addition of danazol, aromatase inhibitors, and anti-human placental P450(arom) monoclonal antibody (mAb3-2C2) in a manner similar to such inhibition in other human tissues. Reverse transcription polymerase chain reaction and Southern blot analysis also revealed P450(arom) mRNA in these tissues. However, neither P450(arom) protein activity nor mRNA was detected in endometrial specimens obtained from normal menstruating women with cervical carcinoma in situ but without any other gynecological disease. These results suggest that at a local level, endometriotic and adenomyotic tissues produce estrogens, which may be involved in the tissue growth through interacting with the estrogen receptor.