THE YEAST HOMOLOG OF U3 SNRNA
THE YEAST HOMOLOG OF U3 SNRNA
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DOI：
10.1002/j.1460-2075.1987.tb02482.x
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发表时间：
1987-07-01
期刊：
影响因子：
11.4
通讯作者：
CESARENI, G
中科院分区：
文献类型：
--
作者：
HUGHES, JMX;KONINGS, DAM;CESARENI, G
snR17是酿酒酵母（Saccharomyces cerevisae）中数量最多的小帽核rna之一，与其他真核生物的U3 snRNA相当。它的长度为328个核苷酸，是其他U3 rna的1.5倍，但在核苷酸序列和预测的二级结构上都具有显著的同源性。人硬皮病核u3rnp特异性抗血清可从酵母核提取物中富集snR17。与其他由单拷贝基因编码的酵母菌snrna不同，snR17是由两个非连锁基因SNR17A和SNR17B编码的。snR17A比snR17B更丰富。除snr17.alpha中snR17B的稳态水平升高外，删除其中一个或另一个基因没有明显的表型影响。——菌株。两个基因缺失的单倍体菌株无法存活，因此酵母U3是必不可少的。
snR17, one of the most abundant capped small nuclear RNAs of Saccharomyces cerevisae, is equivalent to U3 snRNA of other eukaryotes. It is 328 nucleotides in lenght, 1.5 times as long as other U3 RNAs, but shares significant homology both in nucleotide sequence and in predicted secondary structure. Human scleroderma antiserum specific to nuclear U3 RNP can enrich snR17 from sonicated yeast nuclear extracts. Unlike other yeast snRNAs which are encoded by single copy genes, snR17 is encoded by two genetically unlinked genes: SNR17A and SNR17B. The RNA snR17A is more abundant than snR17B. Deleting one or other of the genes has no obvious phenotypic effect, except that the steady-state level of snR17B is increased in snr17.alpha.- strains. Haploid strains with both genes deleted are inviable, therefore yeast U3 is essential.