Virus population dynamics and acquired virus resistance in natural microbial communities
Virus population dynamics and acquired virus resistance in natural microbial communities
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DOI：
10.1126/science.1157358
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发表时间：
2008-05-23
期刊：
影响因子：
56.9
通讯作者：
Banfield, Jillian F.
中科院分区：
文献类型：
--
作者：
Andersson, Anders F.;Banfield, Jillian F.
病毒通过改变宿主的适应性和促进遗传交换来塑造微生物群落的结构和功能。大多数自然生态系统的复杂性阻碍了对病毒-宿主相互作用的详细研究。我们重建病毒和宿主细菌和古细菌的基因组序列从社区基因组数据从两个自然嗜酸生物膜。通过分析在成簇的规则间隔的短回文重复序列（CRISPR）中出现的间隔序列，将病毒与其宿主相匹配，所述CRISPR是病毒抗性的标志。病毒群体基因组分析提供的证据表明，广泛的重组改组序列基序足以逃避CRISPR间隔区。只有最近获得的间隔匹配共存的病毒，这表明，社区的稳定性是通过快速，但补偿性的变化，在主机的电阻水平和病毒种群结构。
Viruses shape microbial community structure and function by altering the fitness of their hosts and by promoting genetic exchange. The complexity of most natural ecosystems has precluded detailed studies of virus- host interactions. We reconstructed virus and host bacterial and archaeal genome sequences from community genomic data from two natural acidophilic biofilms. Viruses were matched to their hosts by analyzing spacer sequences that occur among clustered regularly interspaced short palindromic repeats (CRISPRs) that are a hallmark of virus resistance. Virus population genomic analyses provided evidence that extensive recombination shuffles sequence motifs sufficiently to evade CRISPR spacers. Only the most recently acquired spacers match coexisting viruses, which suggests that community stability is achieved by rapid but compensatory shifts in host resistance levels and virus population structure.