Chromium resistance genetic element flanked by XerC/XerD recombination sites and its distribution in environmental and clinical Acinetobacter strains
Chromium resistance genetic element flanked by XerC/XerD recombination sites and its distribution in environmental and clinical Acinetobacter strains
复制标题
XerC/XerD重组位点侧翼的铬抗性遗传元件及其在环境和临床不动杆菌菌株中的分布
DOI：
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发表时间：
2018
影响因子：
2.1
通讯作者：
M. Petrova
中科院分区：
文献类型：
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作者：
S. Mindlin;A. Petrenko;M. Petrova
在从鲁氏不动杆菌永久冻土菌株中分离出的小质粒中发现了一种新的可移动遗传元件。该元件被命名为 chrAB dif 模块，包含铬抗性基因 chrA 和 chrB，在原始宿主菌株中和转移到贝氏不动杆菌中后均具有功能活性。 3011 bp chrAB dif 模块的两侧是与位点特异性重组系统 dif/Xer 高度同源的 XerC/XerD 重组位点。对公共数据库的分析显示，在主要栖息于环境中的各种不动杆菌属菌株（鲁沃菲不动杆菌、印度不动杆菌、伊德里贾不动杆菌、辛德勒不动杆菌和医院不动杆菌）的不同质粒中，chrAB diff 模块的变体几乎相同。与之前描述的不动杆菌抗生素抗性元件一起，chrAB diff 模块定义了一组新的移动元件，这些元件依赖于 diff/Xer 系统的移动性。我们的观察表明，两侧有不同位点的移动元件的古老起源及其参与具有适应性功能的质粒基因的动员。
A novel mobile genetic element has been identified in small plasmids isolated from permafrost strains of Acinetobacter lwoffii. This element, designated the chrAB dif module, contains the chromium resistance genes chrA and chrB, functionally active both in the original host strains and after transfer into Acinetobacter baylyi. The 3011 bp chrAB dif module is flanked by XerC/XerD recombination sites highly homologous to those of the site-specific recombination system dif/Xer. Analysis of public databases revealed almost identical variants of the chrAB dif module in different plasmids in strains of various Acinetobacter species predominantly inhabiting the environment (A. lwoffii, Acinetobacter indicus, Acinetobacter idrijaensis, Acinetobacter shindleri and Acinetobacter nosocomialis). Together with previously described Acinetobacter antibiotic resistance elements, the chrAB dif module defines a new group of mobile elements that rely on the dif/Xer system for their mobility. Our observations suggest an ancient origin of the mobile elements flanked by dif sites and their participation in the mobilization of plasmid genes bearing adaptive functions.