OLOMOUCINE, AN INHIBITOR OF THE CDC2/CDK2 KINASES ACTIVITY, BLOCKS PLANT-CELLS AT THE G1 TO S AND G2 TO M CELL-CYCLE TRANSITIONS
OLOMOUCINE, AN INHIBITOR OF THE CDC2/CDK2 KINASES ACTIVITY, BLOCKS PLANT-CELLS AT THE G1 TO S AND G2 TO M CELL-CYCLE TRANSITIONS
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DOI：
10.1016/0014-5793(94)01035-8
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发表时间：
1994-10-17
期刊：
影响因子：
3.5
通讯作者：
MEIJER, L
中科院分区：
文献类型：
--
作者：
GLAB, N;LABIDI, B;MEIJER, L
cdc 2/cdk 2蛋白激酶在细胞周期的两个控制点发挥关键作用：GI/S转换和进入有丝分裂。Olomoucine，这些激酶的特异性抑制剂，在两种植物细胞系统中进行了测试：诱导分裂的矮牵牛叶肉原生质体和拟南芥细胞悬浮培养物。从DNA直方图或通过用5-溴脱氧尿苷（BrdUrd）连续标记细胞，然后用双苯甲酰亚胺（Hoechst 33258）和碘化丙啶（PI）双重染色来分析细胞周期状态。这种分析根据DNA复制的程度分辨出几代细胞。Olomoucine显示可逆地阻止诱导分化的矮牵牛细胞在G1期分裂，并使拟南芥细胞在G1晚期和G2期循环。比较aphidicolin，oryzalin和olomoucine的效果表明，在拟南芥细胞悬浮培养，cdc 2/cdk 2样激酶激活的限制点在G1晚期。
The cdc2/cdk2 protein kinases play key roles in the cell cycle at two control points: the GI/S transition and the entry into mitosis. Olomoucine, a specific inhibitor of these kinases, was tested in two plant cell systems: Petunia mesophyll protoplasts induced to divide and Arabidopsis thaliana cell suspension cultures. The cell cycle status was analysed from DNA histograms or through continuous labelling of cells with 5-bromodeoxyuridine (BrdUrd) followed by double staining with bis-benzimide (Hoechst 33258) and propidium iodide (PI). Such analyses resolve cells from several generations according to the extent of their DNA replication. Olomoucine was shown to reversibly arrest differentiated Petunia cells induced to divide at G1 phase and cycling Arabidopsis cells in late G1 and G2. A comparison of the effects of aphidicolin, oryzalin and olomoucine suggests that in the Arabidopsis cell suspension culture, a cdc2/cdk2-like kinase is activated at a restriction point in late G1.