Increase of lycopene production by supplementing auxiliary carbon sources in metabolically engineered Escherichia coli
Increase of lycopene production by supplementing auxiliary carbon sources in metabolically engineered Escherichia coli
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DOI：
10.1007/s00253-011-3091-z
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发表时间：
2011-04-01
影响因子：
5
通讯作者：
Oh, Deok-Kun
中科院分区：
文献类型：
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作者：
Kim, Yeong-Su;Lee, Jae-Hee;Oh, Deok-Kun
在补料分批培养的甘油使用代谢工程菌株的大肠杆菌，补充葡萄糖作为辅助碳源增加番茄红素的生产，由于细胞质量的显着增加，尽管在特定的番茄红素含量减少。补充L-阿拉伯糖增加番茄红素的生产，由于细胞质量和特定的番茄红素含量的增加。补充葡萄糖和l-阿拉伯糖由于两种糖的协同作用而显著增加番茄红素的产生。宿主菌E.杆菌补充L-阿拉伯糖仅刺激番茄红素生物合成的甲羟戊酸途径，补充葡萄糖和L-阿拉伯糖仅协同刺激甲羟戊酸途径。在甘油与10 g l（-1）葡萄糖和7.5 g l（-1）l-阿拉伯糖的补料分批培养中，34 h后的细胞质量、番茄红素浓度、比番茄红素含量和番茄红素生产率分别为42 g l（-1）、1，350 mg l（-1）、32 mg g细胞（-1）和40 mg l（-1）h（-1）。这些值分别是3.9-，7.1-，1.9-和11.7倍，比那些没有辅助碳源，分别高。这是报道的番茄红素的最高浓度和生产率。
In the fed-batch culture of glycerol using a metabolically engineered strain of Escherichia coli, supplementation with glucose as an auxiliary carbon source increased lycopene production due to a significant increase in cell mass, despite a reduction in specific lycopene content. l-Arabinose supplementation increased lycopene production due to increases in cell mass and specific lycopene content. Supplementation with both glucose and l-arabinose increased lycopene production significantly due to the synergistic effect of the two sugars. Cell growth by the consumption of carbon sources was related to endogenous metabolism in the host E. coli. Supplementation with l-arabinose stimulated only the mevalonate pathway for lycopene biosynthesis and supplementation with both glucose and l-arabinose stimulated synergistically only the mevalonate pathway. In the fed-batch culture of glycerol with 10 g l(-1) glucose and 7.5 g l(-1) l-arabinose, the cell mass, lycopene concentration, specific lycopene content, and lycopene productivity after 34 h were 42 g l(-1), 1,350 mg l(-1), 32 mg g cells(-1), and 40 mg l(-1) h(-1), respectively. These values were 3.9-, 7.1-, 1.9-, and 11.7-fold higher than those without the auxiliary carbon sources, respectively. This is the highest reported concentration and productivity of lycopene.