In vivo species specificity of DNA polymerase alpha.
In vivo species specificity of DNA polymerase alpha.
复制标题
DNA聚合酶α的体内物种特异性。
DOI：
10.1007/bf00284700
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Wang,TS
中科院分区：
文献类型：
--
作者：
Francesconi,S;Copeland,WC;Wang,TS
DNA聚合酶a是来自人类、出芽酵母（Saccharomyces cerevisiae）和分裂酵母（Schizosaccharomyces pombe）的同源蛋白，参与染色体DNA的起始和复制。人DNA聚合酶α与s的序列比较。cerevisiaeandS。pombeses的氨基酸序列一致性总体水平分别为32%和34%。我们在这里报道，尽管这三种酶之间的序列保守，但功能活跃的人类DNA聚合酶a在不允许的温度下不能拯救出芽酵母pol1基因的几个不同的条件致死等位基因。此外，人类DNA聚合酶α不能在萌发孢子或营养生长细胞中补充芽殖酵母pol1的空等位基因。在裂变酵母中，功能活跃的人类DNA聚合酶α也不能补充萌发孢子中被破坏的polα::ura4+等位基因。因此，在体内，DNA聚合酶α对染色体DNA的起始和复制具有严格的物种特异性。
The DNA polymerase a enzymes from human, and budding (Saccharomyces cerevisiae) and fission yeast (Schizosaccharomyces pombe) are homologous proteins involved in initiation and replication of chromosomal DNA. Sequence comparision of human DNA polymerase α with that ofS. cerevisiaeandS. pombeshows overall levels of amino acid sequence identity of 32% and 34%, respectively. We report here that, despite the sequence conservation among these three enzymes, functionally active human DNA polymerase a fails to rescue several different conditional lethal alleles of the budding yeastPOL1gene at nonpermissive temperature. Furthermore, human DNA polymerase α cannot complement a null allele of budding yeastPOL1either in germinating spores or in vegetatively growing cells. In fission yeast, functionally active human DNA polymerase α is also unable to complement the disruptedpolα::ura4+allele in germinating spores. Thus, in vivo, DNA polymerase α has stringent species specificity for initiation and replication of chromosomal DNA.