UV photoprotection by combination topical antioxidants vitamin C and vitamin E
UV photoprotection by combination topical antioxidants vitamin C and vitamin E
复制标题
DOI：
10.1067/mjd.2003.425
复制
发表时间：
2003-06-01
影响因子：
13.8
通讯作者：
Pinnell, SR
中科院分区：
文献类型：
--
作者：
Lin, JY;Selim, MA;Pinnell, SR
背景：几乎所有的植物和动物都利用维生素C和E来保护自己免受阳光的伤害.本研究的目的是看看局部维生素C和E的组合是否比同等浓度的局部维生素C或E单独更好地保护皮肤。我们开发了15% L-抗坏血酸（维生素Q）和1% α-生育酚（维生素E）的稳定水溶液。我们每天将抗氧化剂或载体溶液应用于猪皮，持续4天。我们使用带有295 nm带通滤波器的太阳模拟器照射（1- 5倍最小红斑剂量）对照和抗氧化剂处理的皮肤。在第5天，我们测量了抗氧化保护因子，红斑，晒伤细胞，和胸腺嘧啶dimeres.Results：15%的L-抗坏血酸和1% α-生育酚的组合提供了显着的保护，对红斑和晒伤细胞的形成;无论是L-抗坏血酸或1% α-生育酚单独也是保护，但组合是上级。在4天内的应用提供了渐进的保护，产生了4倍的抗氧化保护因子。结论：局部应用维生素C和维生素E可获得明显的光保护作用。我们认为，这些天然产品可以防止皮肤癌和光老化。
Background: Virtually all plants and animals protect themselves from the sun using vitamins C and EObjective. The purpose of this study was to see if a combination of topical vitamins C and E is better for UV protection to skin than an equivalent concentration of topical vitamin C or E alone.Methods. We developed a stable aqueous solution of 15% L-ascorbic acid (vitamin Q and 1% a-tocopherol (vitamin E). We applied antioxidant or vehicle solutions to pig skin daily for 4 days. We irradiated (1-5x minimal erythema dose) control- and antioxidant-treated skin using a solar simulator with a 295-nm band-pass filter. On day 5, we measured antioxidant protection factor, erythema, sunburn cells, and thymine dimers.Results: The combination of 15% L-ascorbic acid and 1% alpha-tocopherol provided significant protection against erythema and sunburn cell formation; either L-ascorbic acid or 1% alpha-tocopherol alone also was protective but the combination was superior. Application during 4 days provided progressive protection that yielded an antioxidant protection factor of 4-fold. in addition, the combination of vitamins C and E provided protection against thymine dimer formation.Conclusion: Appreciable photoprotection can be obtained from the combination of topical vitamins C and E. We suggest that these natural products may protect against skin cancer and photoaging.