Transketolase from Escherichia coli: A practical procedure for using the biocatalyst for asymmetric carbon-carbon bond synthesis
Transketolase from Escherichia coli: A practical procedure for using the biocatalyst for asymmetric carbon-carbon bond synthesis
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DOI：
10.1016/0957-4166(96)00266-2
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发表时间：
1996-08-01
影响因子：
--
通讯作者：
Woodley, JM
中科院分区：
文献类型：
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作者：
Morris, KG;Smith, MEB;Woodley, JM
报道了使用来自大肠杆菌的转酮醇酶进行不对称碳-碳键合成的实用程序。与生物催化剂的反应可以通过使用pH自动滴定仪在无缓冲的水介质中以克级方便地进行。还报道了羟基丙酮酸的改进的大规模合成。版权所有 (C) 1996 爱思唯尔科学有限公司
A practical procedure is reported for the use of the enzyme transketolase, from Escherichia coli, for asymmetric carbon-carbon bond synthesis. The reactions with the biocatalyst are conveniently carried out, on a gram scale, in unbuffered aqueous media by employing a pH autotitrator. An improved large scale synthesis of hydroxypyruvate is also reported. Copyright (C) 1996 Elsevier Science Ltd