Lipid composition of spermatozoa in normozoospermic and asthenozoospermic males
Lipid composition of spermatozoa in normozoospermic and asthenozoospermic males
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DOI：
10.1016/j.plefa.2007.07.001
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发表时间：
2007-07-01
影响因子：
3
通讯作者：
Hosseinipanah, Seyed Mohammad
中科院分区：
文献类型：
--
作者：
Tavilani, Heidar;Doosti, Mahmoud;Hosseinipanah, Seyed Mohammad
前言：脂质在精子的结构和功能活动中起着重要作用。我们调查了弱精子症和正常精子症男性精子的磷脂组成和脂肪酸结合磷脂。患者和方法：对15名弱精子症和8名正常精子症患者的精液样本进行了分析，并分别采用高效薄层色谱法和气相色谱法测定了精子磷脂和脂肪酸。在正常精子和弱精子样本中最丰富的（平均SE）磷脂是磷脂酰乙醇胺（分别为70.9 ± 11.5和44.2 ± 8.5nmol/10（8）个精子）和磷脂酰胆碱（分别为58.6 ± 9.5和34.6 ± 3.2nmol/10（8）个精子）。与正常精子相比，弱精子症患者精子中多不饱和脂肪酸（PUFA; p < 0.01）水平降低，饱和脂肪酸（SFA; p < 0.05）水平升高。(c)2007爱思唯尔有限公司保留所有权利。
Introduction: Lipids play an important role in the structural and functional activity of spermatozoa. We investigated the phospholipids composition and fatty acid-bound phospholipid of spermatozoa from asthenozoospermic and normozoospermic men.Patients and methods: Semen samples were analyzed in 15 asthenozoospermic and eight normozoospermic subjects and the sperm phospholipids and fatty acids were determined using high performance thin layer chromatography and gas chromatography, respectively.Results: The most abundant (mean SE) phospholipids in normozoospermic and asthenozoospermic samples were phosphatidylethanolamine (70.9 +/- 11.5 and 44.2 +/- 8.5nmol/10(8) spermatozoa, respectively) and phosphatidylcholine (58.6 +/- 9.5 and 34.6 +/- 3.2 nmol/10(8) spermatozoa, respectively). Compared to normozoospermic samples, asthenozoospermic samples showed lower levels of polyunsaturated fatty acids (PUFA; p < 0.01) and higher levels of saturated fatty acids (SFA; p < 0.05).Discussion: Changes in content of phospholipids and its fatty acid composition of spermatozoa may be related to infertility in asthenozoospermic males. (c) 2007 Elsevier Ltd. All rights reserved.