An automated MagStation for high-throughput single nucleotide polymorphism genotyping and the dual-color hybridization.
An automated MagStation for high-throughput single nucleotide polymorphism genotyping and the dual-color hybridization.
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DOI：
10.1166/jbn.2009.1066
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发表时间：
2009-10
影响因子：
2.9
通讯作者：
Lan Tian;Song Li;Hongna Liu;Zhifei Wang;N. He
中科院分区：
文献类型：
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作者：
Lan Tian;Song Li;Hongna Liu;Zhifei Wang;N. He
我们设计了一种新型的自动化MagStation，用于使用双色杂交进行高通量单核苷酸多态性（SNP）区分。磁性颗粒（MP）被用作靶DNA载体。本研究中SNP鉴别的原理是使用双色荧光（Cy3，Cy5）探针进行等位基因特异性杂交。MagStation配备了一个96孔自动磁分离装置，当它移动到分离位置时，该装置可以收集试管侧面的MP。MagStation配备了一个加热块，可以在不同的优化温度下进行杂交。该MagStation允许每次测定加热和磁性分离96个样品。使用该自动化MagStation对96个不同样品的亚甲基四氢叶酸还原酶（MTHFR）基因C677T多态性进行半自动化整合。
We designed a novel automated MagStation for high-throughput single nucleotide polymorphism (SNP) discrimination using dual-color hybridization. Magnetic particles (MPs) were used as target DNA carriers. The principle for SNP discrimination in this study was allele specific hybridization using dual-color fluorescent (Cy3, Cy5) probes. The MagStation is equipped with a 96-well automated magnetic separation unit which collects MPs on the side of the tubes as it moves to separation positions. The MagStation equips a heating block, where hybridization at different optimized temperature can be performed. This MagStation permits heating and magnetic separation of 96 samples per assay. The methylenetetrahydrofolate reductase (MTHFR) gene C677T polymorphism of 96 different samples was semi-automated integrated using this automated MagStation.