The Schizosaccharomyces pombe spindle checkpoint protein mad2p blocks anaphase and genetically interacts with the anaphase-promoting complex
The Schizosaccharomyces pombe spindle checkpoint protein mad2p blocks anaphase and genetically interacts with the anaphase-promoting complex
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DOI：
10.1073/pnas.94.15.7965
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发表时间：
1997-07-22
影响因子：
11.1
通讯作者：
Sazer, S
中科院分区：
文献类型：
--
作者：
He, XW;Patterson, TE;Sazer, S
纺锤体检查点监测有丝分裂纺锤体的完整性和着丝粒与纺锤体的附着。在检测到缺陷后，检查点阻断细胞周期进程，从而防止染色体错误分离。在芽殖酵母中的先前研究表明，激活的纺锤体检查点通过未知机制抑制后期的开始。纺锤体检查点的一个可能靶点是后期促进复合物（APC），它控制所有被纺锤体检查点激活阻断的中期后事件。我们已经分离出mad2，纺锤体检查点组件在裂变酵母，并表明，mad2过表达激活检查点，并导致细胞周期停滞在中期到后期的过渡。除了观察到的mad2诱导的逮捕可以部分缓解有丝分裂促进因子失活，我们目前的遗传证据与纺锤体检查点施加细胞周期阻滞抑制APC依赖的蛋白水解的假设一致。
The spindle checkpoint monitors mitotic spindle integrity and the attachment of kinetochores to the spindle. Upon sensing a defect the checkpoint blocks cell cycle progression and thereby prevents chromosome missegregation, Previous studies in budding yeast show that the activated spindle checkpoint inhibits the onset of anaphase by an unknown mechanism. One possible target of the spindle checkpoint is anaphase promoting complex (APC), which controls all postmetaphase events that are blocked by spindle checkpoint activation. We have isolated mad2, a spindle checkpoint component in fission yeast, and shown that mad2 overexpression activates the checkpoint and causes a cell cycle arrest at the metaphase-to-anaphase transition. In addition to the observation that mad2-induced arrest can be partially relieved by mitosis-promoting factor inactivation, we present genetic evidence consistent with the hypothesis that the spindle checkpoint imposes a cell cycle arrest by inhibiting APC-dependent proteolysis.