Visible-light-driven 3-hydroxybutyrate production from acetone and low concentrations of CO<sub>2</sub> with a system of hybridized photocatalytic NADH regeneration and multi-biocatalysts
Visible-light-driven 3-hydroxybutyrate production from acetone and low concentrations of CO<sub>2</sub> with a system of hybridized photocatalytic NADH regeneration and multi-biocatalysts
复制标题
利用混合光催化 NADH 再生和多生物催化剂系统，由丙酮和低浓度 CO<sub>2</sub> 进行可见光驱动的 3-羟基丁酸生产
DOI：
10.1039/d3gc00247k
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发表时间：
2023
期刊：
影响因子：
9.8
通讯作者：
Amao Yutaka
中科院分区：
文献类型：
--
作者：
Kita Yu;Amao Yutaka
聚3-羟基丁酸酯(PHB)衍生塑料具有良好的生物降解性，是解决塑料处置带来的严重环境污染问题的理想材料。以二氧化碳和丙酮为原料，利用可见光催化体系生产新型PHB前体3-羟基丁酸酯，该催化体系由电子供体、光催化染料、电子介体和生物催化剂组成，是解决塑料污染和全球变暖的一种环保途径。在这项工作中，可见光驱动丙酮和低浓度的二氧化碳从气相中捕获的4-(2-羟乙基)-1-哌嗪乙磺酸(HEPES)-NaOH缓冲溶液结合NAD+还原体系的三乙醇胺(TEOA)，水溶性锌卟啉，锌中-四(4-磺基苯基)卟啉四钠盐(ZnTPPS)和Rh配位络合物([CP*Rh(BPY)(H2O)]2+；CP*=五甲基环戊二烯，BPY=2，2‘-联吡啶)，和一个酶提取物包括一个乙酰羧基酶(AC；EC 6.4.1.6)和3-羟基丁酸脱氢酶(3-羟基丁酸脱氢酶；EC 1.1.1.30)是从紫色非硫光合细菌--胶囊红杆菌SB1003(Rb.研究了在含有丙酮和碳酸氢盐的培养液中，厌氧光异养条件下培养的胶囊霉菌SB1003的生长情况。结果表明，直接使用相当于废气15%的二氧化碳作为羧化剂，丙酮可以高产率地转化为3-羟基丁酸酯(60%以上)。此外，还开发了以15%CO2和废丙酮为原料的可见光驱动生产3-羟基丁酸酯。
Poly-3-hydroxybutyrate (PHB)-derived plastics having good biodegradability are attractive materials to solve the issue of serious environmental pollution due to plastic disposal. The production of a new PHB precursor 3-hydroxybutyrate from CO2 and acetone using a visible-light-driven catalytic system consisting of an electron donor, a photocatalytic dye, an electron mediator, and biocatalysts is an environmental approach for both plastic pollution and global warming. In this work, the visible-light driven 3-hydroxybutyrate production from acetone and low-concentration CO2 captured from the gas phase by 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES)–NaOH buffer solution with the combination of a NAD+ reduction system of triethanolamine (TEOA), water-soluble zinc porphyrin, zinc meso-tetra(4-sulfonatophenyl)porphyrin tetrasodium salt (ZnTPPS) and the Rh coordination complex ([Cp*Rh(bpy)(H2O)]2+; Cp* = pentamethylcyclopentadienyl, bpy = 2,2′-bipyridyl), and an enzyme extract including acetone carboxylase (AC; EC 6.4.1.6) and 3-hydroxybutyrate dehydrogenase (HBDH; EC 1.1.1.30) obtained from a purple nonsulfur photosynthetic bacterium, Rhodobacter capsulatus SB1003 (Rb. capsulatus SB1003) cultured in a medium containing acetone and bicarbonate under anaerobic photoheterotrophic conditions was investigated. It was found that acetone can be converted into 3-hydroxybutyrate in high yield (more than 60%) by directly using 15% CO2 equivalent to exhaust gas as a carboxylating agent. Moreover, the development of the visible-light driven 3-hydroxybutyrate production from 15% CO2 and waste acetone as raw materials was also accomplished.