A Method for Murine Islet Isolation and Subcapsular Kidney Transplantation
A Method for Murine Islet Isolation and Subcapsular Kidney Transplantation
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DOI：
10.3791/2096
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发表时间：
2011-04-01
影响因子：
1.2
通讯作者：
Hai, Tsonwin
中科院分区：
文献类型：
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作者：
Zmuda, Erik J.;Powell, Catherine A.;Hai, Tsonwin
自从Ballinger和Reckard的早期开创性工作证明将朗格汉斯胰岛移植到糖尿病啮齿动物体内可以使它们的血糖水平恢复正常以来，胰岛移植一直被认为是一种潜在的1型糖尿病治疗方法(1，2)。最近，人类胰岛移植的进展进一步强化了这一观点(1，3)。然而，两个主要的限制阻碍了胰岛移植成为广泛的临床现实：(A)要求每个患者大量的胰岛，这严重减少了潜在的接受者的数量；(B)需要大量的免疫抑制，这显著影响了儿科患者群体，因为他们容易受到长期免疫抑制的影响。能够克服这些局限性的策略有可能提高胰岛移植的治疗效果。小鼠肾被膜下胰岛移植是一种被广泛接受的模型，可以探索各种改进胰岛移植的策略。本实验要求分离出高质量的胰岛，并将胰岛植入糖尿病受体。这两个过程都需要手术步骤，视频比文本更能展示这些步骤。在这里，我们通过视频和书面协议记录这些过程的详细步骤。我们还简要讨论了不同的移植模式：同基因移植、异基因移植、同基因自身免疫移植和异基因自身免疫移植。
Since the early pioneering work of Ballinger and Reckard demonstrating that transplantation of islets of Langerhans into diabetic rodents could normalize their blood glucose levels, islet transplantation has been proposed to be a potential treatment for type 1 diabetes (1,2). More recently, advances in human islet transplantation have further strengthened this view (1,3). However, two major limitations prevent islet transplantation from being a widespread clinical reality: (a) the requirement for large numbers of islets per patient, which severely reduces the number of potential recipients, and (b) the need for heavy immunosuppression, which significantly affects the pediatric population of patients due to their vulnerability to long-term immunosuppression. Strategies that can overcome these limitations have the potential to enhance the therapeutic utility of islet transplantation.Islet transplantation under the mouse kidney capsule is a widely accepted model to investigate various strategies to improve islet transplantation. This experiment requires the isolation of high quality islets and implantation of islets to the diabetic recipients. Both procedures require surgical steps that can be better demonstrated by video than by text. Here, we document the detailed steps for these procedures by both video and written protocol. We also briefly discuss different transplantation models: syngeneic, allogeneic, syngeneic autoimmune, and allogeneic autoimmune.