Aberrant Development of Enteric Glial Cells in the Colon of Hirschsprung's Disease.
Aberrant Development of Enteric Glial Cells in the Colon of Hirschsprung's Disease.
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先天性巨结肠症结肠肠胶质细胞的异常发育
DOI：
10.3389/fped.2021.746274
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发表时间：
2021
影响因子：
2.6
通讯作者：
Li A
中科院分区：
文献类型：
--
作者：
Zhou T;Liu W;Yu X;Cao Z;Mu W;Hou P;Ren C;Li A
目的：探讨先天性巨结肠（HSCR）不同节段肠神经胶质细胞（EGCs）的发育情况。研究方法：选取35例HSCR患儿结肠标本，采用Western blotting和实时荧光定量PCR方法检测胶质细胞酸性蛋白和S100钙结合蛋白B的相对表达。采用免疫荧光和免疫组织化学方法观察肌间EGCs和神经元细胞在HSCR不同节段的分布。结果如下：胶质细胞酸性蛋白和S100钙结合蛋白B的蛋白和mRNA表达从近端段、扩张段和移行段到无神经节段呈下降趋势（p < 0.05）。免疫荧光和免疫组化显示无神经节、移行和扩张的结肠肌EGCs形态异常，与肌间神经元发育异常一致。结论：HSCR结肠肌间EGCs发育异常，可能影响肠神经元的存活。
Objective: The aim of this study was to explore the development of enteric glial cells (EGCs) in different segments of Hirschsprung's disease (HSCR). Methods: Colonic specimens from 35 children with HSCR were selected to analyze the relative expression of glial fibrillary acidic protein and S100 calcium-binding protein B using Western blotting and real-time fluorescence quantitative PCR. Immunofluorescence and immunohistochemical staining were performed to determine the distribution of myenteric EGCs and neuronal cells in different segments of HSCR. Results: There was a trend of diminished protein and mRNA expression of glial fibrillary acidic protein and S100 calcium-binding protein B from the proximal, dilated, and transitional segments to the aganglionic segment (p < 0.05). Immunofluorescence and immunohistochemistry showed that the EGCs in the aganglionic, transitional, and dilated colonic muscles were morphologically abnormal, which was consistent with the dysplasia of myenteric neurons. Conclusion: Aberrant development of myenteric EGCs was observed in the colon of HSCR, which may affect the survival of enteric neurons.
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影响因子：
24.5
作者：
Aubé, AC;Cabarrocas, J;Neunlist, M
通讯作者：
Neunlist, M
影响因子：
2.4
作者：
Estevao-Costa, Jose;Fragoso, Ana Catarina;Carvalho, Jose Luis
通讯作者：
Carvalho, Jose Luis
DOI：
10.1152/ajpgi.00452.2012
发表时间：
2013-07-01
期刊：
American journal of physiology. Gastrointestinal and liver physiology
影响因子：
--
作者：
Lake JI;Heuckeroth RO
通讯作者：
Heuckeroth RO
影响因子：
2.2
作者：
Tani, Gakuto;Tomuschat, Christian;Puri, Prem
通讯作者：
Puri, Prem
影响因子：
1.8
作者：
Nogueira, A;Campos, M;Carvalho, JL
通讯作者：
Carvalho, JL