Serendipita indica E5′NT modulates extracellular nucleotide levels in the plant apoplast and affects fungal colonization
Serendipita indica E5′NT modulates extracellular nucleotide levels in the plant apoplast and affects fungal colonization
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Serendipita indica E5′NT调节植物细胞质外核苷酸水平并影响真菌定植
DOI：
10.15252/embr.201847430
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发表时间：
2019-02-01
期刊：
影响因子：
7.7
通讯作者：
Zuccaro, Alga
中科院分区：
文献类型：
--
作者：
Nizam, Shadab;Qiang, Xiaoyu;Zuccaro, Alga
细胞外三磷酸腺苷（eATP）是一种重要的信号分子，通过与膜相关受体蛋白的相互作用，介导动物和植物细胞的各种生理过程。eATP调节植物的生长、发育以及对生物和非生物胁迫的反应。它在质外体中的积累诱导ROS产生和细胞质钙增加，介导对入侵微生物的防御反应。我们在这里表明，感知细胞外的核苷酸，如eATP，是重要的植物-真菌的相互作用，并在有益的根内生菌Serendipita indica eATP积累在早期共生阶段的质外体的殖民化。利用液相色谱-串联质谱，细胞学和功能分析，我们表明，S。籼稻分泌SiE 5 'NT，一种能够水解质外体中核苷酸的酶活性外-5'-核苷酸酶。产生细胞外SiE 5 'NT的拟南芥品系显著更好地定殖，具有降低的eATP水平，并且改变了对生物胁迫的响应，表明SiE 5'NT作为相容性因子起作用。我们的数据表明，胞外生物活性核苷酸和它们的感知在真菌-根相互作用中起着重要作用，真菌衍生的酶可以修饰质外体代谢产物以促进真菌的适应。
Extracellular adenosine 5'-triphosphate (eATP) is an essential signaling molecule that mediates different cellular processes through its interaction with membrane-associated receptor proteins in animals and plants. eATP regulates plant growth, development, and responses to biotic and abiotic stresses. Its accumulation in the apoplast induces ROS production and cytoplasmic calcium increase mediating a defense response to invading microbes. We show here that perception of extracellular nucleotides, such as eATP, is important in plant-fungus interactions and that during colonization by the beneficial root endophyte Serendipita indica eATP accumulates in the apoplast at early symbiotic stages. Using liquid chromatography-tandem mass spectrometry, and cytological and functional analysis, we show that S. indica secrets SiE5'NT, an enzymatically active ecto-5'-nucleotidase capable of hydrolyzing nucleotides in the apoplast. Arabidopsis thaliana lines producing extracellular SiE5'NT are significantly better colonized, have reduced eATP levels, and altered responses to biotic stresses, indicating that SiE5'NT functions as a compatibility factor. Our data suggest that extracellular bioactive nucleotides and their perception play an important role in fungus-root interactions and that fungal-derived enzymes can modify apoplastic metabolites to promote fungal accommodation.