Transformation of leguminous plants to study symbiotic interactions
Transformation of leguminous plants to study symbiotic interactions
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DOI：
10.1387/ijdb.130239pr
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发表时间：
2013-01-01
影响因子：
0.7
通讯作者：
Ratet, Pascal
中科院分区：
文献类型：
--
作者：
Iantcheva, Anelia;Mysore, Kirankumar S.;Ratet, Pascal
豆类植物在农业中很重要，因为它们代表了人类和动物消费的重要蛋白质来源。这种高蛋白质含量是由于它们与根瘤菌的共生相互作用而能够利用大气氮作为营养的结果。在分子水平上理解这种相互作用是其在农业中更好地使用以及将其转移到其他作物的长期目标的先决条件。农杆菌介导的转化是研究这种相互作用和揭示通过经典遗传方法发现的不同基因的功能的首选工具。然而，豆科植物往往难以再生和转化。本文介绍了与豆科植物中建立的农杆菌转化相关的技术发展（再生、转化、插入诱变），以及这些研究导致的技术发展或基因发现的不同实例。
Legume plants are important in agriculture because they represent an important source of protein for human and animal consumption. This high protein content results from their capacity to use atmospheric nitrogen for their nutrition as a consequence of their symbiotic interaction with rhizobia. Understanding this interaction at the molecular level is a prerequisite for its better use in agriculture and for the long term objective of its transfer to other crops. Agrobacterium-mediated transformation is a tool of choice for studying this interaction and for unraveling the function of the different genes discovered through classical genetic approaches. However, legume plants are often recalcitrant to regeneration and transformation. This paper describes the technology developments (regeneration, transformation, insertion mutagenesis) related to Agrobacterium transformations that were established in the legume plants, as well as different examples of the technology developments or gene discoveries resulting from these studies.