Passive intranasal monoclonal antibody prophylaxis against murine Pneumocystis carinii pneumonia
Passive intranasal monoclonal antibody prophylaxis against murine Pneumocystis carinii pneumonia
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DOI：
10.1128/iai.70.3.1069-1074.2002
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发表时间：
2002-03-01
影响因子：
3.1
通讯作者：
Harmsen, AG
中科院分区：
文献类型：
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作者：
Gigliotti, F;Haidaris, CG;Harmsen, AG
被动抗体免疫预防是一种用于保护患者免受感染的方法，如果他们无法建立足够的主动免疫反应。局部应用抗体可能对粘膜部位的感染有效。使用卡氏肺囊虫肺炎的SCID小鼠模型，我们能够通过鼻内注射抗体来证明对卡氏肺囊虫空气传播攻击的保护作用。在上述条件下，免疫球蛋白M （IgM）单克隆抗体对小鼠和人类卡氏疟原虫共同的表位减少了99%以上的生物体数量。其中一种IgM单克隆抗体的IgG1开关变体也具有保护作用。这些实验为探索这种方法在保护高危患者免受卡氏弓形虫感染方面的效用提供了一个模型。
Passive antibody immunoprophylaxis is one method used to protect patients against infection if they are unable to mount an adequate active immune response. Topical application of antibody may be effective against infections at mucosal sites. Using a SCID mouse model of Pneumocystis carinii pneumonia, we were able to demonstrate protection against an airborne challenge with P. carinii by intranasal administration of antibody. Immunoglobulin M (IgM) monoclonal antibodies to an epitope shared by mouse and human P. carinii organisms reduced organism numbers by more than 99% under the conditions described. An IgG1 switch variant of one of the IgM monoclonal antibodies was also protective. These experiments provide a model for exploring the utility of this approach in protecting at-risk patients from infection with A carinii.