Morphology of CA3 non-pyramidal cells in the developing rat hippocampus
Morphology of CA3 non-pyramidal cells in the developing rat hippocampus
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DOI：
10.1016/s0165-3806(01)00130-4
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发表时间：
2001-04-30
期刊：
影响因子：
--
通讯作者：
Ben-Ari, Y
中科院分区：
文献类型：
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作者：
Gaïarsa, JL;Khalilov, I;Ben-Ari, Y
虽然一些调查表明，在大鼠海马的本地GABA能电路是功能非常早期的发展，这一结果尚未完成的调查，新生儿interneurons的完整的树突和轴突的分支。在本研究中，细胞内注射biocytin被用来评估在2和6日龄之间的大鼠海马CA3区的中间神经元的分支模式。根据其树突状形态，充满生物细胞素的中间神经元可分为四类：双极、星状、星形和梭形中间神经元。大约一半的生物细胞素填充的新生儿interneurons表现出树突状或躯体丝状伪足的过程。充满的中间神经元的轴突分支广泛分布到CA 3区，在9例中有4例延伸到CA 3区以外的分支进入CA 1区。这些结果表明。尽管还不成熟海马神经元的丝状伪足突起在其靶细胞锥体神经元仍在发育的早期发育良好。这些观察结果与GABA可能在发育早期发挥的营养作用一致。(C)2001 Elsevier Science B.V.保留所有权利。
Although several investigations have shown that the local GABAergic circuit in the rat hippocampus is functional very early in development, this result has not been yet completed by the investigation of the full dendritic and axonal arborization of the neonatal interneurones. In the present study, intracellular injection of biocytin was used to assess the branching pattern of interneurones in the hippocampal CA3 region of rat between 2 and 6 days of age. Based on their dendritic morphology, the biocytin-filled interneurones were divided into four classes: bipolar, stellate, pyramidal-like and fusiform interneurones. About half of the biocytin-filled neonatal interneurones exhibited dendritic or somatic filopodial processes. The axonal arbors of the filled-interneurones were widely spread into the CA3 region, and in four out of nine cases extended beyond the CA3 region to branch into the CA1 region. These results show that. despite immature features. the filopodial processes, the hippocampal interneurones are well developed early in development at a time when their target cells, the pyramidal neurones, are still developing. These observations are consistent with a trophic role that GABA may play early in development. (C) 2001 Elsevier Science B.V. All rights reserved.