The Complexity and Reversibility of the Timer for the Onset of Aggregation in Dictyostelium
The Complexity and Reversibility of the Timer for the Onset of Aggregation in Dictyostelium
复制标题
盘基网柄菌聚集开始计时器的复杂性和可逆性
DOI：
10.1111/j.1432-0436.1980.tb01066.x
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发表时间：
1980
期刊：
影响因子：
2.9
通讯作者：
D. Soll
中科院分区：
文献类型：
--
作者：
J. Mercer;D. Soll
一种简单的条件方法被用来探索在细胞黏液霉菌盘基网柄菌中启动聚集的限速途径或定时器的复杂性。该方法依赖于在其中一个参数(离子强度)变化的两组环境条件下聚集开始所需的时间的不同，并且涉及两组条件之间以短时间间隔的互换。有证据表明，用于聚集开始的定时器由至少两个按顺序操作的组件组成。此外，在定时器的初始分量前进期间，在一个方向上的移动会导致定时器被重置为零。我们的结果还表明，重置计时器会延迟几个聚集相关变化的获得，包括趋化反应、细胞表面的cAMP结合位点和EDTA抗性凝集。
A simple conditional method is used to probe the complexity of the rate limiting pathway, or timer, for the onset of aggregation in the cellular slime mold Dictyostelium discoideum. The method depends upon a difference in the time required for the onset of aggregation under two sets of environmental conditions in which one parameter (ionic strength) is varied, and involves reciprocal shifts between the two sets of conditions at short time intervals. Evidence is presented that the timer for the onset of aggregation is composed of at least two components operating in sequence. In addition, a shift in one direction during the progress of the initial component of the timer causes the timer to be reset to zero. Our results also indicate that resetting the timer delays the acquisition of several aggregation associated changes, including chemotactic responsiveness, cAMP binding sites on the cell surface, and EDTA-resistant agglutination.