Overexpression of RhoA mRNA is associated with advanced stage in testicular germ cell tumour
Overexpression of RhoA mRNA is associated with advanced stage in testicular germ cell tumour
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DOI：
10.1046/j.1464-410x.2001.02030.x
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发表时间：
2001-02-01
影响因子：
4.5
通讯作者：
Yoshida, K
中科院分区：
文献类型：
--
作者：
Kamai, T;Arai, K;Yoshida, K
目的通过检测睾丸生殖细胞肿瘤（GCT）中Rho基因mRNA的表达水平，阐明Rho小GTP结合蛋白（Rho）在GCT进展中的作用。方法对45例日本GCT患者的手术标本中Rho A、Rho B和RhoC基因的mRNA水平进行分析，和来自同一患者的相应的未受影响的组织中，使用逆转录-聚合酶链反应。将肿瘤组织中的表达水平与未受影响的组织中的表达水平进行比较，并评价它们在肿瘤中的表达水平与肿瘤分期之间的关系。结果睾丸肿瘤组织中RhoA的mRNA表达水平明显高于正常睾丸组织（P < 0.01），而RhoB和RhoC的mRNA在两种组织中均未检测到。RhoA mRNA水平的升高与肿瘤分期有关（P < 0.05）。RhoA mRNA在睾丸GCT中的表达与睾丸生殖上皮癌的发生、发展有关，RhoA可能是预测睾丸GCT进展的一个有用指标。
Objective To clarify the role of Rho small GTP-binding protein (Rho) in the progression of testicular germ cell tumour (GCT), by examining the expression levels of mRNAs of Rho genes in testicular GCT.Patients and methods The mRNA levels of the RhoA, RhoB and RhoC genes were analysed in the surgical specimens of testicular GCT tissues from 45 consecutive Japanese patients, and in the corresponding unaffected tissue originating from the same patient, using reverse transcription-polymerase chain reaction. The expression levels in tumour tissues were compared with those in unaffected tissues and the relationship between their expression levels in tumours and tumour stage evaluated. The expression levels of mRNAs of the Rho genes were also evaluated between tumours with seminoma only, and mixed tumours with seminoma and nonseminoma.Results The mRNA levels of RhoA were greater in tumour tissues than in unaffected tissues of the resected testis (P < 0.01); the mRNAs of RhoB and RhoC were not detected in either tissue. The increase in RhoA mRNA levels was related to tumour stage (P < 0.05). The mRNA levels of RhoA in seminomatous and nonseminomatous areas where both were present were higher than those in tumours with seminoma only (P < 0.05).Conclusions These results suggest that RhoA is involved in testicular germinal epithelial carcinogenesis and progression in testicular GCT, indicating that RhoA may be a useful prognostic marker for progression in testicular GCT.