Neuroprotective Effect of Melatonin Against PCBs Induced Behavioural, Molecular and Histological Changes in Cerebral Cortex of Adult Male Wistar Rats
Neuroprotective Effect of Melatonin Against PCBs Induced Behavioural, Molecular and Histological Changes in Cerebral Cortex of Adult Male Wistar Rats
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DOI：
10.1007/s11064-016-2087-6
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发表时间：
2017-02-01
影响因子：
4.4
通讯作者：
Arunakaran, J.
中科院分区：
文献类型：
--
作者：
Bavithra, S.;Selvakumar, K.;Arunakaran, J.
有充分的证据表明多氯联苯（PCBs）是神经毒素。在目前的研究中，我们分析了多氯联苯暴露对成年大鼠的行为影响，并评估了抗氧化褪黑素对多氯联苯作用的同时作用。将大鼠分为4组，分别腹腔注射载药、多氯联苯、多氯联苯+褪黑素和褪黑素，疗程30 d。治疗期结束后，对大鼠进行运动活动和焦虑行为分析。我们通过分子和组织学分析证实了大脑皮层的神经元损伤。我们的数据表明，与对照组相比，多氯联苯处理的大鼠的运动活动和行为受到损害。与多氯联苯治疗的大鼠相比，同时褪黑素治疗的大鼠表现出更强的运动协调能力和更少的焦虑行为。分子和组织学分析支持多氯联苯处理大鼠运动协调功能受损是由于运动皮质区神经退行性变所致。结果证明，褪黑素治疗改善了多氯联苯暴露的成年雄性大鼠的运动协调，减少了焦虑行为，防止了大脑皮层的神经变性。
There is ample evidence stating Polychlorinated biphenyls (PCBs) as neurotoxins. In the current study, we have analyzed the behavioural impact of PCBs exposure in adult rats and assessed the simultaneous effect of antioxidant melatonin against the PCBs action. The rats were grouped into four and treated intraperitoneally with vehicle, PCBs, PCBs + melatonin and melatonin alone for 30 days, respectively. After the treatment period the rats were tested for locomotor activity and anxiety behaviour analysis. We confirmed the neuronal damage in the cerebral cortex by molecular and histological analysis. Our data indicates that there is impairment in locomotor activity and behaviour of PCBs treated rats compared to control. The simultaneous melatonin treated rat shows increased motor coordination and less anxiety like behaviour compared to PCBs treated rats. Molecular and histological analysis supports that, the impaired motor coordination in PCBs treated rats is due to neurodegeneration in motor cortex region. The results proved that melatonin treatment improved the motor co-ordination and reduced anxiety behaviour, prevented neurodegeneration in the cerebral cortex of PCBs-exposed adult male rats.