Nectin-like molecule 1 is a protein 4.1N associated protein and recruits protein 4.1N from cytoplasm to the plasma membrane
Nectin-like molecule 1 is a protein 4.1N associated protein and recruits protein 4.1N from cytoplasm to the plasma membrane
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Nectin 样分子 1 是一种蛋白质 4.1N 相关蛋白，可将蛋白质 4.1N 从细胞质募集到质膜。
DOI：
10.1016/j.bbamem.2005.01.013
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发表时间：
2005-05-20
影响因子：
3.4
通讯作者：
Yuan, JG
中科院分区：
文献类型：
--
作者：
Zhou, Y;Du, GW;Yuan, JG
连接素是免疫球蛋白超家族粘附分子，参与上皮和内皮细胞连接的组织。与脊髓灰质炎病毒受体（PVR/CD155）具有高度同源性。连接蛋白也被命名为脊髓病毒受体相关蛋白（PRRs）。已经鉴定出四种连接蛋白和五种类似连接蛋白的分子。在这里，我们描述了人类和小鼠NECL1样分子的克隆和特性。人和小鼠NECL1在氨基酸水平上具有87.3%的同源性。NECL1在由三个免疫球蛋白样结构域组成的胞外结构域和一个与糖蛋白C和接触蛋白相关的细胞质区同源。RNA印迹和原位杂交分析表明，NECL1主要表达于中枢神经系统，主要表达于包括小脑在内的大脑各区域的神经元细胞体中。体外结合实验证实了NECL1与蛋白4的关联。在。NECL1定位于细胞连接处，通过其c端向质膜募集蛋白4,1n，从而调节细胞连接处的功能。我们认为NECL1和蛋白4.1N复合物参与了神经系统的形态发育、稳定性和动态可塑性。(C) 2005 Elsevier b.v.版权所有
Nectins are immunoglobulin superfamily adhesion molecules (hat participate in the organization of epithelial and endothelial junctions. Sharing high homology with the poliovirus receptor (PVR/CD155). nectins were also named polovirus receptor-related proteins (PRRs). Four nectins and five nectin-like molecules have been identified. Here we describe the cloning and characterization of human and mouse nectin-like molecular 1 (NECL1). Human and mouse NECL1 share 87.3% identity at the amino acid level. NECL1 contains, in ectodomain made of three immunoglobulin-like domains, and a cytoplasmic region homologous to those of glycophorin C and contactin-associated protein. RNA blot and in situ hybridization analysis showed that NECL1 predominantly expressed in the central nervous system, mainly in neuronal cell bodies in a variety of brain regions including the cerebellum. cerebral cortex and hippocampus, In, vitro binding assay proved the association of NECL1 with protein 4. IN. NECL1 localizes to the cell cell junctions and recruits protein 4,1N to the plasma membranes through its C-terminus, thus may regulate the function of the cell cell junction. We propose that the NECL1 and protein 4.1N complex is involved in the morphological development, stability, and dynamic plasticity of the nervous system. (C) 2005 Elsevier B.V All rights reserved.