BACTERIAL METABOLISM OF 3-CHLOROACRYLIC ACID
BACTERIAL METABOLISM OF 3-CHLOROACRYLIC ACID
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DOI：
10.1099/00221287-137-8-2025
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发表时间：
1991-08-01
期刊：
影响因子：
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通讯作者：
DEBONT, JAM
中科院分区：
文献类型：
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作者：
HARTMANS, S;JANSEN, MW;DEBONT, JAM
以3 - 氯丙烯酸（CAA）作为唯一碳源和能源分离出两种细菌菌株。菌株CAA1为洋葱伯克霍尔德氏菌（Pseudomonas cepacia sp.），仅能利用CAA的顺式异构体生长。菌株CAA2为棒状杆菌，能将CAA的两种异构体作为唯一碳源和能源利用。菌株CAA1含有顺式 - CAA水合酶，菌株CAA2含有两种水合酶，一种具有顺式 - CAA水合酶活性，另一种具有反式 - CAA水合酶活性。CAA经水合酶作用的产物是丙二酸半醛。在这两种菌株中，丙二酸半醛随后被一种不依赖辅因子的脱羧酶脱羧，产生乙醛和二氧化碳。
Two bacterial strains were isolated with 3-chloroacrylic acid (CAA) as sole source of carbon and energy. Strain CAA1, a Pseudomonas cepacia sp., was capable of growth with only the cis-isomer of CAA. Strain CAA2, a coryneform bacterium, utilized both isomers of CAA as sole source of carbon and energy. Strain CAA1 contained cis-CAA hydratase and strain CAA2 contained two hydratases, one with cis-CAA hydratase activity and one with trans-CAA hydratase activity. The product of the hydratase activities with CAA was malonate semialdehyde. In both strains malonate semialdehyde was subsequently decarboxylated by a cofactor-independent decarboxylase yielding acetaldehyde and CO2.