Kinetics of SARS-CoV-2 specific IgM and IgG responses in COVID-19 patients.
Kinetics of SARS-CoV-2 specific IgM and IgG responses in COVID-19 patients.
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DOI：
10.1080/22221751.2020.1762515
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发表时间：
2020-12-01
影响因子：
13.2
通讯作者：
Chen, Ling
中科院分区：
文献类型：
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作者：
Sun, Baoqing;Feng, Ying;Chen, Ling
由SARS-CoV-2感染引起的新冠肺炎对全球公共卫生构成严峻挑战。血清抗体检测正成为诊断COVID-19患者的关键方法之一。我们调查了重症监护病房（ICU）和非ICU患者症状发作后对SARS-CoV-2核衣壳（N）和刺突（S）蛋白的IgM和IgG应答。采集了38名COVID-19患者的130份血液样本。ELISA法检测血清N、S蛋白特异性IgM、IgG水平。在同一患者的病程中沿着采集一系列血液样本进行纵向分析，其中包括11例ICU患者和27例非ICU患者。非ICU患者N、S特异性IgM、IgG（N-IgM、N-IgG、S-IgM、S-IgG）在发病后升高。部分非ICU患者的N-IgM和S-IgM在第2周达到高峰，而N-IgG和S-IgG在第3周继续升高。N、S特异性IgM和IgG联合检测可在第一周内检出高达75%的SARS-CoV-2感染者。第3周非ICU患者的S-IgG水平明显高于ICU患者。相比之下，N-IgG在ICU患者中显著高于非ICU患者。非ICU患者血清S-IgG升高与C-反应蛋白（CRP）降低呈正相关。N和S特异性IgM和IgG在症状出现后逐渐升高，可用于SARS-CoV-2感染的检测。分析血清S-IgG的动态变化有助于判断预后。
The emerging COVID-19 caused by SARS-CoV-2 infection poses severe challenges to global public health. Serum antibody testing is becoming one of the critical methods for the diagnosis of COVID-19 patients. We investigated IgM and IgG responses against SARS-CoV-2 nucleocapsid (N) and spike (S) protein after symptom onset in the intensive care unit (ICU) and non-ICU patients. 130 blood samples from 38 COVID-19 patients were collected. The levels of IgM and IgG specific to N and S protein were detected by ELISA. A series of blood samples were collected along the disease course from the same patient, including 11 ICU patients and 27 non-ICU patients for longitudinal analysis. N and S specific IgM and IgG (N-IgM, N-IgG, S-IgM, S-IgG) in non-ICU patients increased after symptom onset. N-IgM and S-IgM in some non-ICU patients reached a peak in the second week, while N-IgG and S-IgG continued to increase in the third week. The combined detection of N and S specific IgM and IgG could identify up to 75% of SARS-CoV-2 infected patients in the first week. S-IgG was significantly higher in non-ICU patients than in ICU patients in the third week. In contrast, N-IgG was significantly higher in ICU patients than in non-ICU patients. The increase of S-IgG positively correlated with the decrease of C-reactive protein (CRP) in non-ICU patients. N and S specific IgM and IgG increased gradually after symptom onset and can be used for detection of SARS-CoV-2 infection. Analysis of the dynamics of S-IgG may help to predict prognosis.