RNA interference-mediated repression of SmCPS (copalyldiphosphate synthase) expression in hairy roots of Salvia miltiorrhiza causes a decrease of tanshinones and sheds light on the functional role of SmCPS
RNA interference-mediated repression of SmCPS (copalyldiphosphate synthase) expression in hairy roots of Salvia miltiorrhiza causes a decrease of tanshinones and sheds light on the functional role of SmCPS
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DOI：
10.1007/s10529-013-1358-4
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发表时间：
2014-02-01
影响因子：
2.7
通讯作者：
Huang, Luqi
中科院分区：
文献类型：
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作者：
Cheng, Qiqing;Su, Ping;Huang, Luqi
丹参酮是丹参中一类具有生物活性的松香烷型去甲二萜醌类化合物。S.丹参（SmCPS）是丹参酮生物合成的第一个关键酶。毛状根;用携带设计用于沉默SmCPS的RNA干扰（RNAi）构建体的发根农杆菌（Agrobacterium rhizogenes）转化丹参，并且我们检测了所得到的SmCPS表达和丹参酮积累。在SmCPS-RNAi毛状根中，与载体对照毛状根相比，SmCPS的转录水平降低至26%，而二氢丹参酮I和隐丹参酮水平降低了53%和38%;未检测到丹参酮IIA。因此，SmCPS表达的降低导致丹参酮水平的降低，证实SmCPS是丹参酮生物合成的关键酶。丹参。
Tanshinones are a group of bioactive abietane-type norditerpenoid quinone compounds in Salvia miltiorrhiza. Copalyldiphosphate synthase of S. miltiorrhiza (SmCPS) is the first key enzyme in tanshinone biosynthesis from the universal diterpene precursor geranylgeranyl diphosphate. Hairy roots of S. miltiorrhiza were transformed with Agrobacterium rhizogenes carrying an RNA interference (RNAi) construct designed to silence SmCPS, and we examined the resulting SmCPS expression and tanshinone accumulation. In SmCPS-RNAi hairy roots, the transcript level of SmCPS was reduced to 26 % while the dihydrotanshinone I and cryptotanshinone levels were decreased by 53 and 38 % compared to those of the vector control hairy roots; tanshinone IIA was not detected. Therefore, the decreased expression of SmCPS caused a decrease in tanshinone levels which verifies that SmCPS is a key enzyme for tanshinone biosynthesis in S. miltiorrhiza.