In vivo tracing of endogenous salicylic acids as the biomarkers for evaluating the toxicity of nano-TiO2 to plants
In vivo tracing of endogenous salicylic acids as the biomarkers for evaluating the toxicity of nano-TiO2 to plants
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内源水杨酸的体内示踪作为评价纳米二氧化钛对植物毒性的生物标志物
DOI：
10.1016/j.aca.2020.10.063
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发表时间：
2021
影响因子：
6.2
通讯作者：
Fang Zhu
中科院分区：
文献类型：
--
作者：
Xu’an Fang;Yuan-Jun Tong;Nan Li;Lu-Dan Yu;Gangfeng Ouyang;Fang Zhu
目前，纳米二氧化钛（nTiO 2）被认为是一种新兴的环境污染物。传统的破坏性和致死性采样方法的瓶颈，nTiO 2的影响，在活的植物很少调查。本文采用固相微萃取（SPME）技术对nTiO_2对植物内源水杨酸的影响进行了定期的活体示踪。在不同nTiO_2含量的土壤中种植芦荟，钛元素在叶片中积累到一定浓度，当nTiO_2含量超过0.1g/kg时，达到最大值（1.1 ± 0.4 μg/g）。水杨酸（SA）和乙酰水杨酸（阿萨）的含量随着nTiO 2的暴露而升高，并与Ti含量呈正相关。此外，随着nTiO 2添加量的增加，丙二醛含量增加，总超氧化物歧化酶含量下降，谷胱甘肽含量沿着波动，表明nTiO 2引起了氧化应激。同时，nTiO 2对芦荟叶片伸长、植株鲜重、根系发育等表观生长指标也有影响，进一步证实了nTiO 2对芦荟的毒性作用。本研究提出了利用水杨酸作为生物标志物来揭示nTiO 2对植物的毒性的可能性，以及其他生物标志物和生物量数据，并且活体SPME技术是监测nTiO 2对植物毒性的有力工具。
Currently, nano-titanium dioxide (nTiO2) is considered an emerging environmental contaminant. Bottlenecked by the traditional destructive and lethal sampling methods, nTiO2’s effect in living plants is poorly investigated. Here,in vivotracing of endogenous salicylic acids at regular intervals was performed by using solid phase microextraction (SPME) technique for evaluating the effects of nTiO2on plants. By planting aloe in soil containing varying amounts of nTiO2, the titanium (Ti) element accumulated in the leaves to concentrations and then reached the maximum of 1.1 ± 0.4 μg/g after nTiO2exceeding 0.1 g/kg. The levels of salicylic acid (SA) and acetylsalicylic acid (ASA) were up-regulated upon the exposure to nTiO2, while were positively correlated to the contents of Ti. Moreover, the increased malondialdehyde, decreased total superoxide dismutase and fluctuated glutathione along with the addition of nTiO2demonstrated the oxidative stress caused by nTiO2. Meanwhile, apparent growth indicators including leaf elongation, plant fresh weight and root development were influenced, which further confirmed the toxicity of nTiO2imparted on aloe. This study presents the possibility of using salicylic acids as biomarkers for revealing the toxicity of nTiO2on plants in addition to the other biomarkers and biomass data, and thein vivoSPME technique is powerful for their monitoring.