MATURATION OF KAINIC ACID SEIZURE BRAIN DAMAGE SYNDROME IN THE RAT .3. POSTNATAL-DEVELOPMENT OF KAINIC ACID BINDING-SITES IN THE LIMBIC SYSTEM
MATURATION OF KAINIC ACID SEIZURE BRAIN DAMAGE SYNDROME IN THE RAT .3. POSTNATAL-DEVELOPMENT OF KAINIC ACID BINDING-SITES IN THE LIMBIC SYSTEM
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DOI：
10.1016/0306-4522(84)90290-2
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发表时间：
1984-01-01
期刊：
影响因子：
3.3
通讯作者：
BENARI, Y
中科院分区：
文献类型：
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作者：
BERGER, ML;TREMBLAY, E;BENARI, Y
[3H]红藻氨酸[KA]结合位点的渐进外观与年龄进行了研究，从大鼠边缘系统的各个区域制备的膜悬浮液，并通过放射自显影。解离速率快的结合位点比解离速率慢的结合位点出现得早。Scatchard分析表明，这两个亚类明显的受体异质性。早在出生后10天就在海马中检测到高亲和力成分，但在杏仁核+梨状叶中仅在第3周结束时发现，此时动物也首次对胃肠外KA作出反应，伴有杏仁核代谢活化的边缘系统癫痫发作。切片放射自显影显示不同的标签海马CA3区出生后第10天。与KA诱导的脑损伤综合征的个体发生的比较表明，高亲和力受体作为KA代谢神经细胞激活的介质的作用。这种受体相互作用本身不会导致对阿蒙角脆弱区域的神经元损伤，这只会发生在杏仁核也被神经毒素激活的年龄。
The progressive appearance of [3H]kainic acid [KA] binding sites with age was studied in membrane suspensions prepared from various regions of the rat limbic system, and by autoradiography. Binding sites with fast dissociation rate appeared earlier than binding sites with slow dissociation rate. Scatchard analysis demonstrated apparent receptor heterogeneity for both subclasses. High affinity components were detected in the hippocampus as early as 10 days after birth, but in the amygdala + piriform lobe were found only towards the end of the 3rd wk, when animals also respond to parenteral KA, for the first time, with limbic seizures accompanied by metabolic activation of the amygdala. Slice autoradiography revealed distinct labelling of the hippocampal CA3 region by postnatal day 10. A comparison with the ontogenesis of the KA-induced seizure-brain damage syndrome suggests a role of high affinity receptors as mediators of metabolic nerve cell activation by KA. This receptor interaction per se does not result in neuronal damage to the vulnerable region of the Ammon''s horn, which will only occur at an age when the amygdala is also activated by the neurotoxin.