HSV-1 clinical isolates with unique in vivo and in vitro phenotypes and insight into genomic differences.
HSV-1 clinical isolates with unique in vivo and in vitro phenotypes and insight into genomic differences.
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HSV-1 临床分离株具有独特的体内和体外表型以及对基因组差异的洞察。
DOI：
10.1007/s13365-016-0485-9
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发表时间：
2017
影响因子：
3.2
通讯作者：
Miller,CraigS
中科院分区：
文献类型：
--
作者：
Danaher,RobertJ;Fouts,DerrickE;Chan,AgnesP;Choi,Yongwook;DePew,Jessica;McCorrison,JamisonM;Nelson,KarenE;Wang,Chunmei;Miller,CraigS
菌株特异性因素以显著但不确定的方式导致单纯疱疹病毒（HSV）复发的可变发生率。需要对这些菌株特异性因子进行研究。在此，我们使用qPCR、体外测定和基因组测序来鉴定独特HSV-1临床分离株的体外和临床表型之间的重要关系。9 HSV-1分离株显示不同的再激活模式的个人进行了研究。与频繁复发性唇疱疹（RHL）相关的分离株（1）在口腔中的病毒脱落率高于与罕见RHL相关的分离株（2）在33 °C下比39 °C下更容易复制。HSV-1分离株在U2 OS细胞中的增殖过程中也表现出比在Vero细胞中更稳定的表型。获得了四个分离株和一个变异株的基因组序列草案，跨越95.6%至97.2%的基因组，全基因组比对表明，这些分离株中的大多数与已知的北美/欧洲分离株聚类。这些发现揭示了可以帮助鉴定与HSV-1分离株相关的独特基因型和表型的程序，这对于确定调节HSV-1再活化的关键病毒因子可能很重要。
Strain-specific factors contribute in significant but undefined ways to the variable incidence of herpes simplex virus (HSV) recrudescence. Studies that investigate these strain-specific factors are needed. Here, we used qPCR, in vitro assays, and genomic sequencing to identify important relationships between in vitro and clinical phenotypes of unique HSV-1 clinical isolates. Nine HSV-1 isolates from individuals displaying varying reactivation patterns were studied. Isolates associated with frequent recurrent herpes labialis (RHL) (1) displayed higher rates of viral shedding in the oral cavity than those associated with rare RHL and (2) tended to replicate more efficiently at 33 °C than 39 °C. HSV-1 isolates also displayed a more stable phenotype during propagation in U2OS cells than in Vero cells. Draft genome sequences of four isolates and one variant spanning 95.6 to 97.2 % of the genome were achieved, and whole-genome alignment demonstrated that the majority of these isolates clustered with known North American/European isolates. These findings revealed procedures that could help identify unique genotypes and phenotypes associated with HSV-1 isolates, which can be important for determining viral factors critical for regulating HSV-1 reactivation.