An unexpected observation concerning the effect of anionic additives on the retention behavior of basic drugs and peptides in reversed-phase liquid chromatography.
An unexpected observation concerning the effect of anionic additives on the retention behavior of basic drugs and peptides in reversed-phase liquid chromatography.
复制标题
关于阴离子添加剂对反相液相色谱中碱性药物和肽保留行为的影响的意外观察。
DOI：
10.1016/j.chroma.2007.03.057
复制
发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Carr,PeterW
中科院分区：
文献类型：
--
作者：
Wang,Xiaoli;Carr,PeterW
具有离子对形成能力的阴离子物种通常用于提高RPLC分离中碱性分析物的保留和效率。然而，很少有人知道有机移动的相改性剂和这样的离子配对阴离子之间的相互作用。在这项工作中，我们测量了添加强无机离子配对阴离子（例如高氯酸盐）后碱性药物和肽的保留增加幅度，作为酸性水-乙腈移动的相中改性剂体积分数的函数在两个不同的固定相上。我们发现，增加保留后，加入各种盐强烈依赖于洗脱剂的强度。在一般情况下，更大的保留增加后，在更高的有机馏分中观察到的阴离子。保留率对有机改性剂体积分数的回归表明，形成阴离子的离子对大大降低了S值，而仅轻微改变[公式：见正文]值。当碱性药物和多肽被加入到移动的相中时，S的减少是其保留增加的主要原因。
Anionic species with ion pair forming ability are commonly used to enhance the retention and efficiency of basic analytes in RPLC separations. However, little is known about the interactions between organic mobile phase modifiers and such ion pairing anions. In this work, we measured the magnitude of the retention increase of basic drugs and peptides upon addition of strong inorganic ion pairing anions (e.g. perchlorate) as a function of the volume fraction of modifier in acidic water-acetonitrile mobile phases on two different stationary phases. We found that the increase in retention upon addition of various salts depended strongly on the eluent strength. In general, larger retention increases upon addition of the anion were observed at higher organic fractions. Regression of retention against the volume fraction of organic modifier indicated that the ion pair forming anions substantially decreased S values while only slightly changing [Formula: see text] values. The decrease in S is the major cause of the retention increase of basic drugs and peptides when such anions are added to the mobile phase.