Molecular typing and genetic characterization of Mycobacterium avium subsp hominissuis isolates from humans and swine in Japan
Molecular typing and genetic characterization of Mycobacterium avium subsp hominissuis isolates from humans and swine in Japan
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DOI：
10.1099/jmm.0.000351
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发表时间：
2016-11-01
影响因子：
3
通讯作者：
Yagi, Tetsuya
中科院分区：
文献类型：
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作者：
Adachi, Takashi;Ichikawa, Kazuya;Yagi, Tetsuya
鸟分枝杆菌hominissuis（MAH）在人类和猪中均引起疾病;然而，MAH分离株的遗传变异尚不清楚。本研究旨在阐明日本人和猪MAH分离株的遗传变异。我们使用鸟分枝杆菌串联重复位点分析了16 S-23 S rDNA内转录间隔区（ITS）序列和可变数目串联重复序列（VNTR），并分析了来自肺MAC（pMAC）疾病患者（n=69）、HIV血清阳性和血培养阳性（HIV-MAC）患者（n=28）和猪（n=23）的MAH分离株ISMav 6和克拉霉素耐药性的患病率。在基于VNTR分析的最小生成树中，猪MAC分离株属于与人pMAC分离株可区分的聚类。来自HIV-MAC的分离株分散在两个集群中。根据16 S-23 S rDNA ITS序列分析，确定了3个主要的分离序列Mav-A、Mav-B和Mav-F，并确定了3个新的分离序列Mav-Q、Mav-R和Mav-S。Mav-A和Mav-F构成了大多数人pMAC菌株;相反，Mav-B在猪分离株中占主导地位。ITS序列在最小生成树中的分布表明，来自不同来源的分离物（即人pMAC、HIV-MAC和猪）具有相似的聚类。这些结果以及ISMav 6携带和克拉霉素耐药性揭示了从人和猪中回收的MAH菌株的遗传多样性。本研究的分子流行病学和遗传特征显示，从人pMAC疾病和猪分离的MAH菌株具有独特的遗传进化谱系。
Mycobacterium avium subsp. hominissuis (MAH) causes disease in both humans and swine; however, the genetic variations in MAH isolates are unclear. The aim of this study was to elucidate the genetic variations in MAH isolates from humans and swine in Japan. We analysed the 16S-23S rDNA internal transcribed spacer (ITS) sequence and variable number of tandem repeats (VNTRs) using the Mycobacterium avium tandem repeat loci, prevalence of ISMav6 and clarithromycin resistance for MAH isolates from patients with pulmonary MAC (pMAC) disease (n=69), and HIV-seropositive and blood culture-positive (HIV-MAC) patients (n=28) and swine (n=23). In the minimum spanning tree based on VNTR analysis, swine MAC isolates belonged to a cluster distinguishable from that of human pMAC isolates. Isolates from HIV-MAC were scattered throughout both clusters. The three major distinct sequevars, Mav-A, Mav-B and Mav-F, were determined according to 16S-23S rDNA ITS sequence analysis in addition to three new sequevars, Mav-Q, Mav-R and Mav-S. Mav-A and Mav-F comprised the majority of human pMAC strains; in contrast, Mav-B predominated in swine isolates. Distribution of ITS sequevars in the minimum spanning tree based on VNTR analysis showed similar clusters of isolates from different origins, i.e. human pMAC, HIV-MAC and swine. These results, together with ISMav6 possession and clarithromycin resistance, revealed the genetic diversity of MAH strains recovered from humans and swine. Molecular epidemiology and genetic characterization in the present study showed the distinctive genetic evolutionary lineage of MAH strains isolated from human pMAC diseases and swine.