Evidence for the Occurrence of a Fast-Acting Inhibitor for Tissue-Type Plasminogen Activator in Human Plasma
Evidence for the Occurrence of a Fast-Acting Inhibitor for Tissue-Type Plasminogen Activator in Human Plasma
复制标题
人血浆中存在组织型纤溶酶原激活剂速效抑制剂的证据
作者：
J. Verheijen;G. Chang;C. Kluft
摘要人血浆中含有一种快速作用的t-PA抑制物，它不同于α-2-抗纤溶酶或α-2-巨球蛋白。该抑制物在正常血浆中的浓度变化很大，远低于已知的血浆蛋白水解酶抑制物的浓度，并且在生理存在的血浆t-PA浓度范围内(0-2IU/ml)。该抑制剂与刀豆蛋白A-琼脂糖结合，加热时相当稳定，不会在优球蛋白组分中沉淀，也可能不是来自血小板。该抑制剂似乎与t-PA形成一个190kD的复合体。这种血浆抑制物与新近发现的内皮细胞抑制物之间的关系尚不清楚。
Summary Human plasma contains a fast-acting t-PA inhibitor, which is not identical with α2-antiplasmin or α2-macroglobulin. The concentration of this inhibitor in normal plasma is highly variable, much lower than that of known plasma protease inhibitors, and in the range of physiologically occurring plasma concentrations of t- PA (0-2 IU/ml). The inhibitor binds to concanavalin A-Sepharose, is rather stable when heated, is not precipitated in euglobulin fractions and probably does not originate from platelets. The inhibitor seems to form a 190 Kd complex with t-PA. The relation between this plasma inhibitor and the recently discovered endothelial cell inhibitor is not yet clear.