Identification of mammalian microRNA host genes and transcription units
Identification of mammalian microRNA host genes and transcription units
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DOI：
10.1101/gr.2722704
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发表时间：
2004-10-01
期刊：
影响因子：
7
通讯作者：
Bradley, A
中科院分区：
文献类型：
--
作者：
Rodriguez, A;Griffiths-Jones, S;Bradley, A
为了获得哺乳动物microRNAs （miRNAs）转录的全局视角，我们注释了这类非编码rna （ncRNAs）在人类和小鼠基因组中的基因组位置和背景。在已知的232个哺乳动物mirna中，我们发现161个与123个定义的转录单位（TUs）重叠。我们在90个具有广谱分子功能的蛋白质编码基因的内含子中，以及66个mrna样非编码rna （mlncRNAs）的内含子和外显子中发现了miRNAs。此外，还发现了基于宿主基因身份的新mirna家族。所有miRNA宿主基因的转录模式从各种来源整理，说明miRNA表达的空间、时间和生理调控。这些发现强烈表明，mirna的转录与宿主转录是平行的，并且这里鉴定的两种不同的mirna转录类别（“外显子”和“内含子”）可能需要略微不同的生物发生机制。
To derive a global perspective on the transcription of microRNAs (miRNAs) in mammals, we annotated the genomic position and context of this class of noncoding RNAs (ncRNAs) in the human and mouse genomes. Of the 232 known mammalian miRNAs, we found that 161 overlap with 123 defined transcription units (TUs). We identified miRNAs within introns of 90 protein-coding genes with a broad spectrum of molecular functions, and in both introns and exons of 66 mRNA-like noncoding RNAs (mlncRNAs). In addition, novel families of miRNAs based on host gene identity were identified. The transcription patterns of all miRNA host genes were curated from a variety of sources illustrating spatial, temporal, and physiological regulation of miRNA expression. These findings strongly suggest that miRNAs are transcribed in parallel with their host transcripts, and that the two different transcription classes of miRNAs ('exonic' and 'intronic') identified here may require slightly different mechanisms of biogenesis.