THE CAENORHABDITIS-ELEGANS GENES CED-3 AND CED-4 ACT CELL AUTONOMOUSLY TO CAUSE PROGRAMMED CELL-DEATH
THE CAENORHABDITIS-ELEGANS GENES CED-3 AND CED-4 ACT CELL AUTONOMOUSLY TO CAUSE PROGRAMMED CELL-DEATH
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DOI：
10.1016/0012-1606(90)90174-h
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发表时间：
1990-03-01
影响因子：
2.7
通讯作者：
HORVITZ, HR
中科院分区：
文献类型：
--
作者：
YUAN, JY;HORVITZ, HR
Ced-3和Ced-4基因的突变可以防止秀丽线虫发育过程中发生的几乎所有程序性细胞死亡。为了确定这两个基因的作用部位，我们进行了遗传镶嵌分析。我们培育了携带Ced-3(+)或Ced-4(+)的线虫动物，在其他纯合子Ced-3或Ced-4遗传背景中携带Ced-3(+)或Ced-4(+)。我们使用与复制有关的其他基因作为标记，以识别在某些但不是所有细胞中存在复制的遗传马赛克动物。这些马赛克动物的细胞死亡幸存者的模式表明，CED-3和CED-4的产物都在死亡细胞内发挥作用，导致细胞死亡。
Mutations in the genes ced-3 and ced-4 prevent almost all of the programmed cells deaths that occur during Caenorhabditis elegans development. To determine the sites of action of these two genes, we performed genetic mosaic analyses. We generated C. elegans animals that carried a free chromosomal duplication bearing either ced-3(+) or ced-4(+) in an otherwise homozygous ced-3 or ced-4 genetic background. We used other genes on the duplication as markers to identify genetic mosaic animals in which the duplication was present in some but not all cells. The patterns of cell death survivors in these mosaic animals indicated that the products of both ced-3 and ced-4 function within dying cells to cause cell death.