DIFFERENTIATION OF LENS-LIKE STRUCTURES FROM NEWT IRIS EPITHELIAL-CELLS INVITRO
DIFFERENTIATION OF LENS-LIKE STRUCTURES FROM NEWT IRIS EPITHELIAL-CELLS INVITRO
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DOI：
10.1073/pnas.71.12.5052
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发表时间：
1974-01-01
影响因子：
11.1
通讯作者：
WATANABE, K
中科院分区：
文献类型：
--
作者：
EGUCHI, G;ABE, SI;WATANABE, K
分离的有色虹膜上皮细胞在单层培养2~3周后密集生长。在滞后期，许多细胞出现脱色现象。当接种后5-6周，培养物融合，许多细小的晶状体样结构(30-70个/皿)从致密的无色素性上皮细胞灶分化而来。这些晶状体样结构出现在所有来源于虹膜腹侧和背侧细胞的培养物中。通过免疫学和超微结构技术鉴定这些结构为晶状体。
Dissociated cells of pigmented iris epithelium from adult newts grew intensively in monolayer cultures after a lag of two to three weeks. During the lag period, depigmentation occurred in many cells. When cultures became confluent five to six weeks after seeding, many tiny lens-like structures (30-70 per plate) differentiated from dense foci of amelanotic epithelial cells. These lens-like structures appeared in all cultures originated from cells of ventral as well as dorsal iris. The identification of these structures as lens was established by both immunological and ultrastructural techniques.