Aminoguanidine reversal of the inhibitory effects of ornithine analogs on the in vitro clonogenic survival of the R3327AT prostate-derived tumor.
Aminoguanidine reversal of the inhibitory effects of ornithine analogs on the in vitro clonogenic survival of the R3327AT prostate-derived tumor.
复制标题
氨基胍逆转鸟氨酸类似物对 R3327AT 前列腺源性肿瘤体外克隆存活的抑制作用。
DOI：
10.1016/0304-3835(81)90098-7
复制
发表时间：
1981
期刊：
影响因子：
9.7
通讯作者：
W. Fair
中科院分区：
文献类型：
--
作者：
W. Heston;D. W. Lazan;W. Fair
在哥本哈根大鼠前列腺源性肿瘤细胞系的体内研究中，这些细胞系的鸟氨酸脱羧酶（ODC）活性对α-二氟甲基鸟氨酸（α-DFMO）抑制的敏感性与正常前列腺组织相同。ODC抑制剂α-甲基鸟氨酸和α-二氟甲基鸟氨酸可抑制肿瘤在小牛血清中的体外生长。这种抑制作用可被二胺氧化酶抑制剂氨基胍部分逆转。此外，α-DFMO在缺乏二胺氧化酶活性的血清中对这些细胞的生长没有抑制作用，但当加入二胺氧化酶时具有抑制作用。
In in vivo studies with the Copenhagen rat prostrate-derived tumor cell lines, the activity of ornithine decarboxylase (ODC) of these lines were as sensitive to inhibition by α-difluoromethylornithine (α-DFMO) as normal prostatic tissue. The in vitro growth of the tumor in calf serum was inhibited by ODC inhibitors α-methylornithine and α-difluoromethylornithine. This inhibition was partially reversed by the diamine oxidase inhibitor amino-guanidine. Further, α-DFMO was not inhibitory to the growth of these cells in sera which lacked diamine oxidase activity, but was inhibitory when diamine oxidase was added.