MicroRNA-315-5p promotes rice black-streaked dwarf virus infection by targeting a melatonin receptor in the small brown planthopper
MicroRNA-315-5p promotes rice black-streaked dwarf virus infection by targeting a melatonin receptor in the small brown planthopper
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MicroRNA-315-5p通过靶向小褐飞虱中的褪黑素受体促进水稻黑条矮缩病毒感染
DOI：
10.1002/ps.6410
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发表时间：
2021
影响因子：
4.1
通讯作者：
Xu Qiufang
中科院分区：
文献类型：
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作者：
Zhang Jianhua;Dong Yan;Wang Man;Wang Haitao;Yi Dianshan;Zhou Yijun;Xu Qiufang
MicroRNAs（miRNAs）是一类内源性小分子非编码RNA，在多种生物学过程中发挥重要作用。大多数植物病毒通过昆虫媒介传播。我们利用水稻黑条矮缩病毒（RBSDV）和小褐飞虱（SBPH）互作系统研究了miR-315 - 5 p在植物病毒感染昆虫中的调控作用。我们的研究结果表明，miR-315 - 5 p在2龄若虫中的表达水平最高，并且在SBPH的唾液腺和中肠中高表达。在SBPH中，miR-315 - 5 p响应并调节RBSDV感染。注射miR-315 - 5 p模拟物agomir-315显著增加RBSDV蓄积，而注射miR-315 - 5 p抑制剂agomir-315减少SBPH中的病毒蓄积。此外，通过双荧光素酶报告基因测定，褪黑激素受体被鉴定为miR-315 - 5 p的靶基因。褪黑素受体的敲除显著增加了RBSDV外壳蛋白基因S10和复制相关基因S5 - 1、S6和S9 - 1的表达。此外，褪黑激素受体拮抗剂luzindole和激活剂阿戈美拉汀治疗显着增加和减少RBSDV积累SBPH，分别。与对照相比，miR-315 - 5 p不影响SBPH中RBSDV获取的效率。然而，注射miR-315后，RBSDV的传播效率显著降低。结论综上所述，我们的数据显示miR-315 - 5 p通过直接靶向褪黑素受体而有利于RBSDV在其昆虫载体中的感染。这些发现为miRNAs在病毒-昆虫载体相互作用中的功能提供了新的见解。© 2021化学工业协会。
BACKGROUNDMicroRNAs (miRNAs), a class of small non‐coding endogenous RNAs, play key roles in various biological processes. Most plant viruses are transmitted by insect vectors. However, little is known about the function of miRNAs on plant virus‐insect host interaction.RESULTSWe investigated the role of miR‐315‐5p in regulation of plant viral infection in insects using a rice black‐streaked dwarf virus (RBSDV) and small brown planthopper (SBPH) interaction system. Our results showed that miR‐315‐5p had the highest expression level in 2nd‐instar nymph, and was highly expressed in the salivary gland and midgut in SBPH. miR‐315‐5p was in response to and regulated RBSDV infection in SBPH. Injection of miR‐315‐5p mimic, agomir‐315, significantly increased the RBSDV accumulation, whereas injection of miR‐315‐5p inhibitor, antagomir‐315, reduced virus accumulation in SBPH. Furthermore, a melatonin receptor was identified as a target gene of miR‐315‐5p by the dual luciferase reporter assay. Knockdown of the melatonin receptor significantly increased the expression of RBSDV coat protein geneS10and replication related genes,S5‐1,S6, andS9‐1. Furthermore, treatment with melatonin receptor antagonist luzindole and activator agomelatine significantly increased and reduced RBSDV accumulation in SBPH, respectively. Compared to the control, miR‐315‐5p did not affect the efficiency of RBSDV acquisition in SBPH. However, the efficiency of RBSDV transmission was significantly reduced after injecting antagomir‐315.CONCLUSIONTaken together, our data reveal that miR‐315‐5p is beneficial for RBSDV infection in its insect vector by directly targeting a melatonin receptor. These findings provide a new insight to the function of miRNAs in virus‐insect vector interaction. © 2021 Society of Chemical Industry.