Induction of osteoclast-like cells derived from the synovial lavage fluids of patients with temporomandibular joint disorders
Induction of osteoclast-like cells derived from the synovial lavage fluids of patients with temporomandibular joint disorders
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DOI：
10.1016/j.joca.2006.08.001
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发表时间：
2007-03-01
影响因子：
7
通讯作者：
Takahashi, T.
中科院分区：
文献类型：
--
作者：
Takano, H.;Ariyoshi, W.;Takahashi, T.
目标：虽然生化研究已经检查了颞下颌关节（TMJ）疾病（TMD）患者的滑液（SF），骨破坏和重建的分子机制的细节仍然未知。在这项研究中，我们诱导和特点的破骨细胞样细胞从颞下颌关节紊乱病患者的SF，并调查这些细胞的参与，在发病机制中的TMD.Methods：我们收集SF细胞从颞下颌关节紊乱病患者泵程序后，培养破骨细胞样细胞，并检查其特性，包括破骨细胞标志物和骨吸收活动。此外，我们还从TMD患者的SF中通过连续传代培养获得成纤维细胞。使用这些成纤维细胞，我们使用免疫细胞化学染色检查成纤维细胞标志物，并分析核因子-κ B配体（RANKL）mRNA水平的受体激活剂。结果：用重组人巨噬细胞集落刺激因子（M-CSF）和1，25-二羟基维生素D-3 [1，25（OH）（2）D-3]或前列腺素E-2（PGE（2））诱导TMD患者SF细胞产生破骨细胞样细胞。这些多核巨细胞抗酒石酸酸性磷酸酶（TRAP）阳性，并具有吸收骨的能力。TMD患者SF的成纤维细胞成纤维细胞标志物阳性，RANKL mRNA表达上调。结论：TMD患者关节浸润的SF细胞在以进行性骨破坏或骨重建为特征的TMD疾病的发病机制中起重要作用。(C)2006年国际骨关节炎研究学会。由爱思唯尔有限公司出版。保留所有权利。
Objective: Although biochemical studies have examined the synovial fluid (SF) of patients with temporomandibular joint (TMJ) disorders (TMDs), the details of the molecular mechanism of bone destruction and remodeling remain unknown. In this study, we induced and characterized osteoclast-like cells from the SF of patients with TMD and investigated the participation of these cells in the pathogenesis of TMD.Methods: We collected SF cells from patients with TMD after a pumping procedure, cultured osteoclast-like cells, and examined their characteristics, including osteoclast markers and bone resorption activities. In addition, we obtained fibroblastic cells from the SF of TMD patients by continuous sub-culturing. Using these fibroblastic cells, we examined fibroblast markers using immunocytochemical staining and analyzed the receptor activator of nuclear-factor-kappa B ligand (RANKL) mRNA levels. Detection of soluble form of RANKL (sRANKL) in the SF was measured by enzyme-linked immunosorbent assay (ELISA).Results: Osteoclast-like cells were induced from the SF cells of patients with TMD by adding recombinant human (rh) macrophage colony stimulating factor (M-CSF) and either 1,25-dihydroxy vitamin D-3 [1,25(OH)(2)D-3] or prostaglandin E-2 (PGE(2)). These multinucleated giant cells were positive for tartrate-resistant acid phosphatase (TRAP) and had the ability to absorb bone. The fibroblastic cells from the SF of TMD patients were positive for fibroblast markers and RANKL mRNA was up-regulated. Detection of sRANKL in SF of patient group was significantly higher than control group.Conclusion: The results suggest that the joint-infiltrating SF cells from TMD patients play important roles in the pathogenesis of these disorders, which is characterized by progressive bone destruction or remodeling. (C) 2006 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.