Microfluidic bolus induced gradient generator for live cell signalling
Microfluidic bolus induced gradient generator for live cell signalling
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DOI：
10.1007/s10404-012-1125-1
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发表时间：
2013-07-01
影响因子：
2.8
通讯作者：
Roy, Partha
中科院分区：
文献类型：
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作者：
Ramji, Ramesh;Roy, Partha
细胞生物学中的主要事件是由配体与细胞表面受体的结合和/或其转运到细胞中引发的。我们提出了一个简单的微通道系统，集成了一个团发生器和表面粘附的细胞培养域的研究。我们的系统允许将生物分子的小包或大团递送到细胞群。由于推注的压力驱动的微流体流动，沿着微通道的长度沿着建立了细胞表面结合配体的梯度。表皮生长因子（EGF）结合其受体的A431细胞的实验数据。我们突出了改变Peclet数（或流速），丸形状，丸体积和配体浓度上的梯度纵向形成的微通道的效果。建立了描述配体与细胞表面受体的瞬时对流、扩散、分散和结合的数学模型。该模型为我们的系统提供了必要的设计指导方针，与实验数据具有良好的定性一致性。结果表明，方法来调节结合配体的量和梯度独立。与现有的微流体系统相比，这种简单的微系统适合产生涉及更大细胞群的更长范围梯度。
Major events in cell biology are initiated by the binding of ligands to cell surface receptors and/or their transport into cells. We present a study of a simple microchannel system that integrates a bolus generator and surface-adhered cell culture domains. Our system allows the delivery of small packets or boluses of biomolecules to a cell population. Owing to pressure driven microfluidic flow of the bolus, a gradient of cell surface bound ligands is established along the length of the microchannel. Experimental data for the epidermal growth factor (EGF) binding to its receptor on A431 cells are presented. We highlight the effect of changing Peclet number (or flowrate), bolus shape, bolus volume and ligand concentration on the gradient formed longitudinally in the microchannel. A mathematical model describing the transient convection, diffusion, dispersion and binding of ligands to cell surface receptors is developed. The model provides essential design guidelines for our system with good qualitative agreement with experimental data. The results suggest ways to modulate the amount of bound ligand and the gradient independently. This simple microsystem is suitable for generating longer range gradients involving larger cell populations as compared to existing microfluidic systems.