Properties and detection of two cryptoviruses from pepper (Capsicum annuum)
Properties and detection of two cryptoviruses from pepper (Capsicum annuum)
复制标题
DOI：
10.1007/s11262-011-0634-4
复制
发表时间：
2011-10-01
期刊：
影响因子：
1.6
通讯作者：
Valverde, Rodrigo A.
中科院分区：
文献类型：
--
作者：
Sabanadzovic, Sead;Valverde, Rodrigo A.
辣椒（Capsicum annuum L.）含有一系列类似于隐病毒基因组的内源性dsRNA分子。在这项工作中，我们已经完成了辣椒隐病毒1（PCV-1）的分子特征，从CV“Jalapeo M”和产生的另一个隐病毒的完整基因组序列从CV“匈牙利蜡”命名为辣椒隐病毒2（PCV-2）。这两种病毒在两个基因组片段中共享有限的相同氨基酸含量，并且在遗传学上看起来更接近于从其他作物报道的隐病毒（即萝卜隐病毒3，黑树莓隐病毒）而不是彼此。利用两套病毒特异性引物对辣椒叶片和种子中的两种病毒进行了RT-PCR检测。这两种病毒检测到在几个辣椒品种测试，无论是作为单一或混合感染。
Pepper (Capsicum annuum L.) contains a range of endogenous dsRNA molecules resembling the genomes of cryptoviruses. In this work, we have completed the molecular characterization of Pepper cryptic virus 1 (PCV-1) from cv "Jalapeo M" and generated complete genomic sequences of another cryptovirus from cv "Hungarian Wax" designated Pepper cryptic virus 2 (PCV-2). The two viruses share limited identical amino acid content in both genomic segments and appear phylogenetically closer to cryptoviruses reported from other crops (i.e. Raphanus sativus cryptic virus 3, Black raspberry cryptic virus) than to each other. Two sets of virus-specific primers were successfully used in RT-PCR tests for the simultaneous and discriminative detection of these two viruses in pepper leaves and seeds. Both viruses were detected in several pepper cultivars tested, either as single or mixed infections.