Structural abnormalities of subicular dendrites in subjects with schizophrenia and mood disorders -: Preliminary findings
Structural abnormalities of subicular dendrites in subjects with schizophrenia and mood disorders -: Preliminary findings
复制标题
DOI：
10.1001/archpsyc.57.4.349
复制
发表时间：
2000-04-01
影响因子：
--
通讯作者：
Dwork, AJ
中科院分区：
文献类型：
--
作者：
Rosoklija, G;Toomayan, G;Dwork, AJ
背景：对精神分裂症患者脑下丘的尸检研究发现，锥体细胞体积变小，树突状细胞微管相关蛋白2的免疫反应减弱。虽然这些发现表明精神分裂症患者的锥体下细胞树突可能发生结构改变，但这种可能性尚未被直接测试。方法：采用快速高尔基体浸渍的方法，比较精神分裂症患者(n=13)和心境障碍患者(n=6)与非精神疾病患者(n=8)的锥体下树突状细胞的形态特征。这些样本由不知道诊断的医生进行处理和分析。用Sholl法观察下丘脑和梭形回内树突的分布情况。结果：椎体下锥体细胞树突棘密度和树突分枝与诊断组间有显著相关性。精神分裂症和情绪障碍组的脊柱密度明显低于非精神病组。在情绪障碍的病例中，脊柱密度降低显然与重大精神疾病的强烈家族史有关。诊断组对非顶束下树突的Sholl分析和对梭形回新皮质锥体细胞树突的Sholl分析均无显著影响。结论：我们观察到精神分裂症与主要的心境障碍和顶下树突的结构异常有关。需要进一步的研究，以在更大的样本中测试这种联系，并评估家族史和混杂因素的潜在作用，如药物和慢性住院。
Background: Postmortem studies of the subiculum from subjects with schizophrenia have detected smaller pyramidal cell bodies and diminished immunoreactivity for the dendritic protein, microtubule-associated protein 2. While these findings suggest that subicular pyramidal cell dendrites may be structurally altered in subjects with schizophrenia, this possibility had not been tested directly.Methods: Rapid Golgi impregnation of archival brain specimens was used to compare the morphologic characteristics of subicular dendrites in subjects with schizophrenia (n = 13) and mood disorders (n = 6) with subjects without psychiatric disease (n = 8). The specimens were processed and analyzed by physicians blind to diagnosis. The extent of dendritic trees in the subiculum and fusiform gyrus was examined by Sholl analysis. Spine density on apical dendrites of subicular pyramidal cells was determined at a fixed distance from the cell body.Results: Spine density and arborization of subicular apical dendrites were significantly related to diagnostic group. Spine density was significantly lower in the schizophrenia and mood disorder groups than in the nonpsychiatric group. Among the mood disorder cases, diminished spine density was apparently related to a strong family history of major psychiatric diseases. There were no significant effects of diagnostic group on Sholl analysis of nonapical subicular dendrites nor on Sholl analysis of dendrites of neocortical pyramidal cells in the fusiform gyrus.Conclusions: We have observed an association between schizophrenia and major mood disorders and structural abnormalities of subicular apical dendrites. Further studies are needed to test this association in a larger sample and to evaluate the potential role of family history and of confounding factors, such as medications and chronic institutionalization.