Cellular distribution of uranium after acute exposure of renal epithelial cells:: SEM, TEM and nuclear microscopy analysis
Cellular distribution of uranium after acute exposure of renal epithelial cells:: SEM, TEM and nuclear microscopy analysis
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DOI：
10.1016/j.nimb.2005.01.069
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发表时间：
2005-04-01
影响因子：
1.3
通讯作者：
Khodja, H
中科院分区：
文献类型：
--
作者：
Carrière, M;Gouget, B;Khodja, H
据报告，铀接触的主要健康影响是化学性肾毒性，主要在近端小管细胞中观察到功能和组织学损害。铀以铀酰-碳酸氢盐或铀酰-柠檬酸盐络合物的形式进入近端小管。我们的研究目的是探讨铀的毒性机制，细胞内积累和再分配后，急性中毒的大鼠肾近端小管上皮细胞，作为其化学形式的函数，显微镜观察急性暴露后的肾上皮细胞显示存在细胞内沉淀物的存在下，作为细针的铀磷酸盐定位在细胞溶酶体已公布。然而，沉淀物形成的初始位置尚未确定：它们可以在内化之前在细胞外形成，也可以直接在细胞内形成。铀溶解度作为培养基的ICP-MS分析的函数和初始浓度。在平行，铀的吸收和分布在细胞单层暴露于U-碳酸氢盐进行了研究，核微探针分析。最后，通过扫描电子显微镜观察（SEM）检测铀沉淀物的存在，而通过透射电子显微镜（TEM）在细胞的薄切片上观察细胞外和/或细胞内沉淀物。(c)2005 Elsevier B. V.保留所有权利。
The major health effect of uranium exposure has been reported to be chemical kidney toxicity, functional and histological damages being mainly observed in proximal tubule cells. Uranium enters the proximal tubule as uranyl-bicarbonate or uranyl-citrate complexes. The aim of our research is to investigate the mechanisms of uranium toxicity, intracellular accumulation and repartition after acute intoxication of rat renal proximal tubule epithelial cells, as a function of its chemical form.Microscopic observations of renal epithelial cells after acute exposure to uranyl-bicarbonate showing the presence of intracellular precipitates as thin needles of uranyl-phosphate localized in cell lysosomes have been published. However the initial site of precipitates formation has not been identified yet: they could either be formed outside the cells before internalization, or directly inside the cells.Uranium solubility as a function and initial concentration was specified by ICP-MS analysis of culture media. In parallel, uranium uptake and distribution in cell monolayers exposed to U-bicarbonate was investigated by nuclear microprobe analyses. Finally, the presence of uranium precipitates was tested out by scanning electron microscopic observations (SEM), while extracellular and/or intracellular precipitates were observed on thin sections of cells by transmission electron microscopy (TEM). (c) 2005 Elsevier B.V. All rights reserved.