THE FLIA GENE ENCODING SIGMA(28) IN YERSINIA-ENTEROCOLITICA
THE FLIA GENE ENCODING SIGMA(28) IN YERSINIA-ENTEROCOLITICA
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DOI：
10.1128/jb.177.9.2299-2304.1995
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发表时间：
1995-05-01
影响因子：
3.2
通讯作者：
CORNELIS, GR
中科院分区：
文献类型：
--
作者：
IRIARTE, M;STAINIER, I;CORNELIS, GR
小肠结肠炎耶尔森菌是一种引起胃肠道综合征的肠杆菌。其致病性取决于70 kb pYV质粒的存在，该质粒指导Yop分泌。由YscA-U和LcrD蛋白组成的Yop分泌机制与其他细菌中表征的鞭毛组装机制存在一些结构相似性。鞭毛组装需要sigma（28），一个替代的sigma因子。lcrD基因的上游区域类似于sigma识别的启动子（28），表明Yop分泌和鞭毛组装之间的相似性可以扩展到它们的调节。Y.小肠结肠炎菌还含有致病性决定簇，例如编码Myf抗原亚单位的myfA。myfA的启动子区也类似于sigma识别的启动子（28）。试图澄清; sigma（28）在lcrD、myfA和鞭毛基因表达中的作用，我们克隆了编码sigma（28）同源物的fliA基因，并对其进行了诱变。小肠结肠炎与其他细菌一样，fliA是运动所必需的。然而，它既不参与纤维合成，也不参与Yop分泌。fliA突变使我们能够监测运动在发病机制中的作用。至少在小鼠模型中，运动似乎不是Y。小肠结肠炎发病机制
Yersinia enterocolitica is an enterobacterium responsible for gastrointestinal syndromes. Its pathogenicity depends on the presence of the 70-kb pYV plasmid, which directs Yop secretion. The Yop secretion machinery, consisting of the YscA-U and LcrD proteins, presents some structural similarity with the flagellum assembly machinery characterized in other bacteria. Flagellum assembly requires sigma(28), an alternative sigma factor. The region upstream of the lcrD gene resembles promoters recognized by sigma(28), suggesting that the similarity between Yop secretion and flagellum assembly could extend to their regulation. The chromosome of Y. enterocolitica also contains pathogenicity determinants such as myfA, which encodes the Myf antigen subunit. The promoter region of myfA also resembles promoters recognized by sigma(28). In an attempt to clarify; the role of sigma(28) in the expression of lcrD, myfA, and flagellar genes, we cloned, sequenced, and mutagenized the fliA gene encoding the sigma(28) homolog in Y. enterocolitica. As is the case in other bacteria, fliA was required for motility. However, it was involved neither in fibrilla synthesis nor in Yop secretion. The fliA mutant allowed us to monitor the role of motility in pathogenesis. At least in the mouse model, motility seemed not to be required for Y. enterocolitica pathogenesis.