EVIDENCE THAT NEBULIN IS A PROTEIN-RULER IN MUSCLE THIN-FILAMENTS
EVIDENCE THAT NEBULIN IS A PROTEIN-RULER IN MUSCLE THIN-FILAMENTS
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DOI：
10.1016/0014-5793(91)80503-u
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发表时间：
1991-05-06
期刊：
影响因子：
3.5
通讯作者：
TRINICK, J
中科院分区：
文献类型：
--
作者：
LABEIT, S;GIBSON, T;TRINICK, J
获得了大量肌原纤维蛋白星云蛋白的部分氨基酸序列。 这由约35个残基的重复基序和7 X 35 = 245个残基的超重复序列组成。 重复基序很可能主要是α-螺旋，并在细丝中与肌动蛋白和原肌球蛋白相互作用。 来自不同物种的星云蛋白被发现大小与细丝长度成比例。 这些数据与星云蛋白作为蛋白质统治者调节精确细丝组装的提议一致。
Partial amino acid sequence was obtained from the massive myofibrillar protein nebulin. This consists of repeating motifs of about 35 residues and super-repeats of 7 x 35 = 245 residues. The repeat-motifs are likely to be largely alpha-helical and to interact with both actin and tropomyosin in thin filaments. Nebulin from different species was found to vary in size in proportion to filament length. The data are consistent with the proposal that nebulin acts as a protein-ruler to regulate precise thin filament assembly.