Design Variations for an Aptamer-Based DNA Nanodevice
Design Variations for an Aptamer-Based DNA Nanodevice
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DOI：
10.1166/jbn.2005.007
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发表时间：
2005-03-01
影响因子：
2.9
通讯作者：
Simmel, Friedrich C.
中科院分区：
文献类型：
--
作者：
Beyer, Stefan;Dittmer, Wendy U.;Simmel, Friedrich C.
基于凝血酶结合DNA适体的DNA纳米器件的性能强烈依赖于蛋白质结合核心序列的化学修饰和延伸。我们在这里给出了一个概述的影响，荧光标记和延伸的适体装置序列上的结合特性。在5'末端上的序列延伸以及在5'或3'末端上的标记不会显著降低结合能力。3'端的序列延伸完全抑制结合。该知识可用于构建可作为可切换分子信标操作的双标记适体装置。
The performance of DNA nanodevices based on a thrombin-binding DNA aptamer is strongly dependent on chemical modifications and extensions of the protein-binding core sequence. We here give an overview of the influence of fluorescent labeling and extension of the aptamer device sequence on its binding properties. Sequence extension on the 5' end as well as labeling on the 5' or 3' end does not significantly reduce the binding capabilities. Sequence extension on the 3' end completely suppresses binding. This knowledge can be utilized for the construction of a doubly labeled aptamer device which can be operated as a switchable molecular beacon.